单唾液酸四己糖神经节苷脂缓释微球保护大鼠脊髓继发性损伤的实验研究

Protective Effects of Monosialoganglioside Microspheres Administrated Locally in Secondary Spinal Cord Injury

目的:探讨经蛛网膜下腔给予单唾液酸四己糖神经节苷脂(GM-1)缓释微球保护大鼠脊髓继发性损伤的增强效应及其机制。方法:188只成年SD大鼠随机分为对照组8只,模型组60只,普通GM-1组60只和GM-1微球组60只。采用Nys- trom法制备脊髓压迫损伤模型(50g×5min),术后24,72,168和336 h检测大鼠运动功能、脑脊液中GM-1含量,术后8,24,72,168和336 h检测大鼠脊髓组织内SOD活力和MDA含量,以及脊髓Caspase-3表达情况。结果:与模型组比较,GM-1微球组大鼠BBB运动功能评分高,脑脊液中GM-1含量多,脊髓组织内SOD活力强和MDA含量少,Caspase-3表达明显,具有显著统计学差异(P<0.01),亦优于GM-1组,但无统计学差异。结论:蛛网膜下腔给予GM-1缓释微球对脊髓继发性损伤的保护作用更强,主要机制涉及抗自由基损伤和抗细胞凋亡。

To investigate the promotion effects and the protective mechanism of monosialoganglioside, GM-1 , PLGA sustained-release microspheres on secondary spinal cord injury in rats by subarachnoid medication route. Method： The one hundred and eighty-eight adult Sprague Dawley rats were randomly divided into 4 groups： control group （8 rats）, model group （60 rats）, normal GM-1 group （60 rats） and GM-1 microspheres group （60 rats）. The spinal cord injury model （50 g×5 min） was induced by Nystroms method, the function of spinal cord was evaluated by BBB score, and the amount of GM-1 in the cerebrospinal fluid at 24, 72, 168 and 336 h after SCI were detected. The activity of SOD, the amount of MDA and the expression of Caspase-3 in spinal cord tissue at 8, 24, 72, 168 and 336 h after SCI were detected. Result： Compared with the model group, in the GM-1 microspheres group： the BBB score was higher, the amount of GM-1 in the cerebrospinal fluid was more, the amount of MDA in spinal core was less, and the activity of SOD, the expression of Caspase-3 were stronger （ P〈0.01 ）. The GM-1 microspheres group was better than the normal GM- 1 group also, but it was no statistical significance. Conclusion： The protective effects of GM-1 PLGA microspheres was stronger on the secondary spinal cord injury, the mechanism included anti free radicals injury and anti apoptotic effect.