HPLC法测定牛黄清火丸中大黄素和大黄酚的含量

Determination the content of Emodin and Chrysopimnoi in Niuhuangqinghuo pills by HPLC

目的:建立牛黄清火丸的含量测定方法。方法:采用高效液相色谱法。色谱柱:SHIMADZU VP-ODS(250 mm×4.6 mm,5μm),流动相:甲醇-0.1%磷酸溶液(80:20,V/V),流速:1.0ml·min^(-1),检测波长:254 n nm,柱温:30℃。结果:大黄素在10.04～50.20μg·ml^(-1)范围内线性关系良好(r=0.999 8);平均加样回收率为98.0%,RSD=1.5%(n=6);大黄酚在20.04～60.12μg·ml^(-1)范围内线性关系良好(r=1.0000);平均加样回收率为99.2%,RSD 1.1%(n=6)。结论:该方法简便易行,结果准确可靠,可适用于牛黄清火丸的质量控制。

To establish a HPLC method for determination the content of emodin and chrysophanol in Niuhuangqinghuo pills.＇Method： The shimadzu VP-ODS C18 column（250 mm ×4.6 mm,5μm） was used, the mobile phase was methanol-0.1% phos phoric acid solution（80：20）, the column temperature was 30℃, the detection wavelength was 254 nm. the flow rate was 1.0 ml· min^-l Result： The linear ranges of emodin and chrysophanol were at 10.04 - 50.20 μg（r=0.999 8 ） and 20.4 - 60.12 μg· ml^-1（r=1.000 0） respectively, the average recoveries （n=6） were 98.0% and 99.2% with RSD 1.5 % and 1.1% respectivery. Conclusion： The method is simple and accurate, it can be used for quality control of Niuhuangqinghuo pills.