穴位埋线对氯氮平大鼠下丘脑单胺类神经递质的影响

Effects of Acupoint Catgut Implantation on Hypothalamic Monoamine Neurotransmitters in Rats Treated with Clozapine

【目的】观察穴位埋线对氯氮平大鼠下丘脑单胺类神经递质的影响。【方法】将SD大鼠36只随机分为埋线组、氯氮平组和空白对照组。除空白对照组外,其他2组大鼠均灌胃氯氮平,由5 mg.kg-1.d-1渐增至30 mg.kg-1.d-1,而后递减至22 mg.kg-1.d-1,并维持至实验结束。服用氯氮平60 d后埋线组选取两组穴位:①百会、心俞(双)、曲池(双);②大椎、脾俞(双)、足三里(双),交替埋线,每5 d治疗1次,共12次。观察各组大鼠治疗前后体质量变化,并检测下丘脑单胺类神经递质多巴胺(DA)和5-羟色胺(5-HT)的含量。【结果】长期服用氯氮平可使大鼠体质量显著性增加,DA和5-HT含量显著性降低(均P<0.05或P<0.01),而穴位埋线治疗可显著性抑制大鼠体质量的增加,升高下丘脑中DA和5-HT的含量(P<0.05)。【结论】穴位埋线减轻氯氮平副作用的机制可能与其能升高下丘脑内单胺类神经递质的含量有关。

[ Objective] To explore the effect of acupoint catgut implantation （ACI） on hypothalamic monoamine neurotransmitters in rats treated with clozapine. [ Methods ] Thirty-six SD rats were randomized into ACI group, clozapine group and blank control group. Except the blank control group, the rats in other two groups received clozapine. The dosage of clozapine for the rats was 5 mg·kg^-1·d^-1 at the first time, increased to 30 mg·kg^-1·d^-1, and then reduced to 22 mg·kg^-1·d^-1 which maintained till the end of the experiment. After 60-day treatment with clozapine, ACI group was treated with ACI alternatively on Baihui （GV20）, bilateral Xinshu （BL15） and bilateral Quchi （ LI11 ） , or on Dazhui （GV14） , bilateral Pishu （BL20） , bilateral Zusanli （ ST36）, one time every 5 days, 12 times in total. The changes of body weight before and after treatment, and the hypothalamic monoamine neurotransmitters contents of dopamine （DA） and 5-hydroxytryptamine （5-HT） were observed in the three groups. [ Results ] In the clozapine group, the rats body weight markedly increased, and the contents of DA and 5-HT decreased （P 〈0. 05 or P 〈0. 01 compared with the blank control group）. ACI decreased the body weight and increased the contents of DA and 5-HT in clozapine group （P 〈 0. 05）. [ Conclusion ] The mechanism of ACI reduc!ng the side effects of clozapine is probably related to the increase of hypothalamic monoamine neurotransmitters contents.