加味四逆散对心理应激损伤大鼠海马nNOS表达的影响

Effect of Modified Sini Powder on nNOS Expression in Hippocampus of Rats with Psychological-Stress-Induced Damage

【目的】观察调肝治法方药——加味四逆散对慢性心理应激损伤大鼠海马神经元神经型一氧化氮合酶(nNOS)表达的影响。【方法】取W istar大鼠24只随机分为3组:正常组(不施加任何刺激)、模型组(给予21 d的随机应激刺激)、加味四逆散组(每次刺激前1 h灌胃中药1次,剂量为3.38 g/只)。正常组及模型组每次灌胃等容积生理盐水。采用免疫组织化学方法检测nNOS表达。【结果】3组大鼠海马的CA3区锥体细胞均有nNOS表达,而模型组表达的强度显著高于正常组,其阳性细胞数及阳性累积分显著性增多(P<0.01);加味四逆散可显著性降低大鼠海马nNOS表达,减少其阳性细胞数及阳性累积分(P<0.01)。【结论】加味四逆散改善慢性心理应激的作用与其能抑制大鼠海马神经元nNOS的高表达,从而避免NO升高损害细胞有关。

[ Objective] To observe the effect of Modified Sini Powder （MSP） on the expression of hippocampal neuronal nitric oxide synthase （nNOS） in rats with chronic psychological stress. [ Methods ] Twenty-four Wistar rats were randomized into 3 groups： normal group, model group （receiving random stress stimulation for 21 days）, and MSP group （receiving gastric infusion of MSP 3.38 g for each rat one hour before stimulation）. The normal group and the model group received the same volume of saline. The expression of hippocampal nNOS was detected with immunohistochemical method. [ Results] nNOS expression was found in neuronal pyramidal cells of hippocampus CA3 area in the three groups. The expression was obvious, positive cell count and its scoring were increased in the model group as compared with the normal group （ P 〈 0. 01 ）, and MSP reduced the nNOS expression as well as positive cell count and its scoring in rats with chronic stress （P 〈 0.01 ）. [ Conclusion ] MSP can relieve the damage induced by chronic stress in rats, and its mechanism is probably related with the highexpression of nNOS in hippocampus, thus avoid the increase of NO damaging neuronal cells.