白木香种子的超低温保存研究

Study on Cryopreservation of Seeds of Aquilaria sinensis(Lour.)Gilg.

【目的】探讨白木香种子长期保存的方法。【方法】将不同含水量的白木香种子放置在液氮罐(-196℃)中保存55 d后取出,测定其发芽率,并考察冷冻方法对其发芽率的影响。【结果】含水量为16.30%的种子发芽率完全丧失;含水量为13.02%、9.34%的种子发芽率大大降低;而含水量为7.35%的种子仍能保持较高的发芽率。速冷的白木香种子比缓冻的发芽率高。【结论】含水量为7.35%的白木香种子置于液氮中保存为一种安全、有效的长期保存方法。

[ Objective] To explore the long-term storage method for the seeds of Aquilaria sinensis （Lour.） Gilg.. [ Methods] The seeds of Aquilaria sinensis （Lout.） Gilg. with different water contents were preserved with liquid nitrogen for 55 days. And then the seeds were taken out to detect their germination capacity. The effect of cryopreservation methods on the germination capacity was also observed. [ Results ] The seeds with water content being 16. 30% lost their germination capacity completely, the seeds with water content being 13.02% and 9.34% had a lower germination capacity, and the seeds with water content being 7.35% had a higher germination capacity. Quickly-frozen seeds had a higher germination capacity than the slowly-frozen seeds. [ Conclusion] Cryopreservation with liquid nitrogen is suitable for the long-term preservation of the seeds of Aquilaria sinensis （Lour.） Gilg. with water content being 7.35%.