碱性成纤维细胞生长因子与CD44V6基因在甲状腺癌中的表达及意义

Alteration of Expression of CD44V6 and bFGF and Their Clinical Significance

目的研究转移相关基因CD44V6与bFGF在甲状腺癌组织中的表达及其临床意义。方法应用免疫组化SP方法测定60例甲状腺组织中CD44V6与bFGF的阳性表达率,分析其阳性率与肿瘤转移的关系。结果CD44V6表达阳性率在淋巴结转移组(23/27)明显高于无淋巴结转移组(16/33)(P<0.01)。在乳头状癌中的表达率为75.0%,滤泡状腺癌组为71.4%,在髓样癌中及未分化癌中未见阳性表达。bFGF表达阳性率在淋巴结转移组(18/27)明显高于无淋巴结转移组(10/33)(P<0.01)。在乳头状癌中的表达率为75.0%,滤泡状腺癌组为28.6%,在未分化癌组为40.0%,髓样癌中未见阳性表达。分析CD44V6阳性表达与临床分期的关系,发现随分期的增加而增加,CD44V6的表达阳性率在Ⅰ、Ⅱ、Ⅲ期分别为64.9%,75.0%,100.0%。结论CD44V6表达与甲状腺癌的颈淋巴结转移密切相关;与临床分期,病理类型有一定关系。

Objective To investigate the expression of CD44V6 and bFGF gene in 60 patients with thyroid carcinoma from september,2005 to november,2006. Methods The expressions of CD44V6 and bFGF gene in 60 specimens of thyroid cancer tissues were determined by immunochemical S - P method, there were 24 males and 36 females. The clinical stages were stages I in 37, I] in 12, m in 4, V in 7. Among 60 cases, 27 were lymph node metastasis and 33 non-metastasis;pathological patterns were papillary type in 32, acinous in 21, medullary in 2 and non- differential in 5. Restilts The positive rate of expression of CD44V6 gene was 23/27（85.2%） in group of lymph node metastasis, 16/33 （30.3%） in group of non - metastasis（ Х^2 = 8.79, P 〈 0.01 ） ;24/32（75.0% ） in group of papillary carcinoma, 15/ 21（71.4% ） in group of acinous carcinoma.The expression of CD44V6 was negative in the groups of medullary （2 cases） ,non-differential carcinoma （5 cases） and normal tissue of thyroid （10 cases）. The positive rate of CD44V6 was 64.9%, 75.0% and 100.0% in the clinical stages Ⅰ,Ⅱ and Ⅲ, respectively. The positive rate of expression of bFGF gene was 18/27（66.7%） in group of lymph node metastasis, 10/33（30.3%） in group of non - metastasis （ χ^2 = 7.89, P 〈 0.01 ） ; 24/32 （75.0 % ） in group of papillary carcinoma, 6/21 （28.6 % ） in group of acinous carcinoma. The expression of bFGF was negative in the groups of medullary （2 cases）, normal tissue of thyroid （10 cases）. Conclusion The results suggested that the expression of CD44V6 were closely correlated with lymph node metastasis, clinical stages and pathological pattems.