转甜菜碱醛脱氢酶基因玉米的田间筛选及生理分析

Screening in Field and Physiological Analysis on Transgenic Maize with Betaine Aldehyde Dehydrogenase Gene

为了对已获得的转甜菜碱醛脱氢酶基因(BADH)玉米3个株系的后代进行田间耐盐性鉴定,采用稀释1倍的人工海水在温室内模拟田间盐胁迫进行处理,结果表明:在盐胁迫条件下,转BADH基因玉米成活率较对照高,转基因株系的叶片电导率极显著地低于对照,而叶绿素含量极显著地高于对照。转基因株系后代的耐盐性与对照材料相比具有明显的优势,验证了在田间盐胁迫条件下,BADH基因在转基因玉米后代中的表达,同时也为培育转BADH基因玉米纯系奠定基础。

In this study, betaine aldehyde duced stress with artificial sea water in than non-transformed maize. The results salt stress was lower than that of control after salt stress. dehydrogenase gene （BADH） transformed maize was examined for tolerance to salt ingreenhouse. The results indicated that BADH transgenie lines had higher salt tolerance of physiological detection showed that the electrical conductivity of transgenic line after significantly. The transgenic progeny had higher chlorophyll content than that of control after salt stress.