银杏叶提取物对氧自由基的影响

Effect of Ginkgo biloba Extract on Reducing Oxygen Free Radicals

分别提取银杏叶脂溶性成分和水溶性成分,采用NBT(氯化硝基四氮唑蓝)光还原法检测银杏叶提取物消除氧自由基能力的大小,并计算出抑制率。结果表明:银杏叶脂溶性成分和水溶性成分均能消除氧自由基,水溶性成分消除氧自由基的能力强于脂溶性成分。水溶性提取物组的活力单位是0.167mg,脂溶性提取物NaOH组的活力单位是0.17mg,脂溶性提取物无水乙醇组的活力单位是0.795mg。

Liposoluble constituents and water-soluble constituents of Ginkgo biloba leaf were extracted and NBT （Nitrpotetrazolium bluechloride） photoreduction method was adopted to examine the reducing abilities on oxygen radicals, and the change of absorption number after reaction was observed and the restrain rate was computed. The results indicated that liposoluble constituents and water-soluble constituents of ginkgo leaves could reduce oxygen free radicals. The ability to reduce oxygen free radicals for water-soluble substance is better than that of liposoluble constituents. The active unit of water-soluble constituents is 0.167 mg, that of liposoluble constituents NaOH group is 0.17 mg, that of liposoluble constituents absolute alcohol is 0.795 mg.