两种取材方法快速诊断流感病毒感染的对比研究

Contrast Study of Different Collection Methods to Detection of Influenza Virus Infection

目的比较咽部分泌物咽拭子取材和负压吸引鼻导管取材的优越性。方法采用咽拭子和负压吸引鼻导管两种方法取咽部分泌物,分别用GICA法、免疫荧光法检测30例急性呼吸道感染患者的咽部分泌物中的流感抗原。结果30例急性呼吸道感染者中GICA法检出流感A阳性3例、B阳性2例;免疫荧光法检出A、B阳性各3例,其中5例与GICA法相同。GICA法中,咽拭子取材检出流感A、B阳性各2例;鼻导管取材检出流感A阳性3例,B阳性2例,两种取材方法阳性检出率相比,差异无统计学意义(P>0.05)。结论咽拭子取材法检测流感抗原可信、可靠且较负压吸引鼻导管法方便快捷。

Objective To compare the superiority of different collection methods for pharynx nasalis secretion by throat swab and nasal catheter. Methods Pharynx nasalis secretion of 30 patients with acute respiratory tract infection was tested via throat swab and nasal catheter by means of gold immunochromatography assay （ GICA ） and immunofluorescence assay （ IFA ）. Results No obvious difference in positive rate was found between the two collection methods for pharynx nasalis secretion. Conclusion The collection methods by throat swab is rapid in the detection of influenza virus antigen.