固本化痰通脉方含药血清对大鼠血管平滑肌细胞增殖及细胞周期素D_1的影响

Effect of Guben Huatan Tongmai Recipe on Expression of Cyclin D_1 and the Proliferation of Vascular Smooth Muscle Cells Caused by PDGF-BB Stimulation in Rat

目的:观察固本化痰通脉方对大鼠血管平滑肌细胞生长增殖、细胞周期及细胞周期素D1表达的影响,以探讨其作用机制。方法:血小板源生长因子BB(PDGF-BB)刺激体外培养的大鼠血管平滑肌细胞使其快速增殖生长,加入不同浓度的固本化痰通脉方含药血清作用一定时间后,通过MTT法测定其对血管平滑肌细胞生长的抑制率,流式细胞技术测定细胞周期及细胞周期素D1的表达。结果:固本化痰通脉方能明显抑制PDGF-BB刺激的血管平滑肌细胞增殖,还以浓度依赖方式减少S期细胞、阻滞细胞于G0/G1期,并下调细胞周期素D1的表达。结论:固本化痰通脉方能抑制血管平滑肌细胞的增殖生长,阻滞细胞周期的进行,因而可能具有抗动脉粥样硬化的作用,其作用机理可能与调控血管平滑肌细胞细胞周期素D1的活性有关。

Objective：To investigate the effect of Guben Huatan Tongmai Recipe（GHTR） on the proliferation, cell cycle and eyclin DI activity of vascular smooth muscle cells（VSMCs） in rat in order to find out the mechanism of its action. Methods： In this study, cell culture technique was used in vitro. VSMCs stimulated by platelet derived growth factor BB （PDGF-BB） were treated by GHTR in different concentration for a certain period, then the inhibitory ratio of cell proliferation was measured by MTT, cell cycle distribution and cyclin DI activity of the cells were observed by FlowCytometry respectively. Results： GHTR could obviously inhibit the proliferation of VSMCs stimulated by PDGF-BB, and it could also decrease S phase cells, increase G0/G1 phase cells and down-regulate cyclin D1 activity of VSMCs in concentration-dependent fashion, and the results were significant compared with the normal serum plus PDGF-BB-treated. Conclusion： GHTR could obviously inhibit the proliferation of VSMCs and may prevent atherosclerosis, which mechanism may be realized by regulating cyclin D1 activity of VSMC.