甘蔗宿根矮化病诊断方法比较研究

Comparison of Diagnostic Methods for Sugarcane Ratoon Stunting Disease

甘蔗宿根矮化病(Ratoon stunting disease,RSD)是我国的一种主要甘蔗病害,没有明显的外部症状,我国常用的诊断方法是内部症状检查。为了探明内部症状(分为边部维管束和中部维管束)诊断的可靠性及实际应用价值,应用准确度高、灵敏度好的斑点酶标免疫试验(Dot blot enzyme immunoassays,DB-EIA)与之比较。结果表明:边部维管束法与DB-EIA检测阳性一致率为30.4%(7/23)、阴性一致率为73.3%(11/15);中部维管束法与DB-EIA检测比较,阳性一致率为50%(5/10),阴性一致率为75%(6/8);2种内部症状诊断的准确性没有明显差异,但中部维管束法诊断准确性略高于边部维管束法。这一结果表明,与DB-EIA相比,内部症状诊断可靠性较差,是一种相当粗略的诊断方法,诊断结果仅能参考。

Sugarcane Ratoon stunting disease （RSD） is known as an economically important disease in China. It is difficult to be diagnosed in the field because of the absence of visible symptoms, and its diagnosis is usually based on interior symptom observation of basal node of mature stalks （ ISO）. This study compared the detection results between diagnostic method of RSD with ISO and Dot blot enzyme immunoassays （ DB - EIA） with high accuracy and sensitivity, in order to investigate the reliability and practical application value of diagnostic technique of RSD with ISO. The results showed that positive and negative consistency rates of the detection results were 30.4% （7/23） and 73.3% （11/15） , respectively, comparising to the diagnostic method of RSD with colonized vascular bundles on longitudinal section of basal node of mature stalks split by quarter （CVBQ） and DB - EIA. The positive and negative consistency rates of the detection results were 50% （5/ 10） and 75 % （6/8） , respectively, comparising to the diagnostic technique with colonized vascular bundles on longitudinal section of basal node of mature stalks split by half （CVBH） and DB - EIA. There was not significant difference between CVBH and CVBQ, but the reliability of CVBH was better than that of CVBQ. It was concluded that ISO was relatively unreliable, and its detection results were only for reference, comparising with DB - EIA.