摘要
以中华蜜蜂(Apis cerana cerana)和意大利蜜蜂(Apis mellifera ligustica)王浆为材料,用改进的苯酚-氯仿法提取蜂王浆中的DNA,利用35个随机引物对提取出来的DNA进行RAPD扩增,结果表明:有6个随机引物能扩增出谱带,其中1个引物对所有王浆DNA样品的扩增结果完全相同,5个引物出现多态性。共检测到32条扩增谱带,其中24条为多态带。
The experiment used royal jelly of Apis cerana and Apis mellifera as materials, extracted the DNA of royal jelly with modified phenol -chloroform method, the extracted DNA was analyzed by RAPD with 35 arbitrary primers. The results showed that： in the 35 primers that were tested, I obtained the same bands. 5 were polymorphous, a total of 32 bands were obtained, of which 24 were polymorphic.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2007年第4期631-633,共3页
Acta Agriculturae Universitatis Jiangxiensis
基金
江西省自然科学基金资助项目(0530031)
