α_1受体阻断剂对慢性心力衰竭大鼠心肌神经生长因子表达的影响

Terazosin prevents the decrease of beta-nerve growth factor in myocardium of rats with heart failure

目的评价α1受体阻断剂(特拉唑嗪)对慢性心力衰竭(CHF)大鼠心肌神经生长因子(NGF)表达水平的影响。方法选24只雄性Wistar大鼠,应用阿霉素腹腔注射的方法制作CHF模型;随机分成特拉唑嗪治疗组(DCM-T)和安慰剂治疗组(DCM-P),DCM-T组接受特拉唑嗪灌胃治疗,0.4mg/kg·d,DCM-P组用淀粉代替特拉唑嗪,疗程6周;RT-PCR和Western blot方法评价心肌NGF表达水平。结果DCM-P组大鼠心肌NGFmRNA和蛋白表达水平比正常对照组明显下降(51.7%vs45.0%,均P<0.01);DCM-T组NGFmRNA和蛋白下降值明显低于DCM-P组(35.0%vs51.7%和21.0%vs45.0%,均P<0.05)。结论CHF大鼠心肌NGF表达水平明显下降;特拉唑嗪治疗能够阻止CHF大鼠心肌NGF下调,有助于改善CHF大鼠心肌交感神经重构。

[Objective] To investigate the effect of alpha 1 adrenoceptor blocker （Terazosin） on the expression of beta-nerve growth factor in myocardium of rats with heart failure. [Methods] Thirty adult male Wistar rats were randomly divided into DCM group （n =24） and normal control group （NC, n =6）. Then DCM group was randomly divided into DCM-T group （n =12） and DCM-P group （n =12）; Adfimycin was injected by intrapefitoneal to rats of the DCM group at a dose of 2 mg/kg for 10 times. Terazosin was used to treat the heart failure at a dose of 0.4 mg/kg per day in DCM-T group, while as a placebo, starch was used to treat the heart failure at the same dose in DCM-P group; Nerve growth factor mRNA was measured with RT-PCR; Nerve growth factor protein was measured with Western Blot [Results] Compared with NC group, NGF mRNA and protein were significant decreased in DCM-P group （51.7% and 45.3%, all P 〈0.01）; NGFmRNA and protein were less decreased in DCM-T group than in DCM-P group （35.0% vs 51.7% and 21.8% vs 45.0%, all P 〈0.05）. [Conclusions] bNGF were significant decreased in the myocardium of heart failure rats; Terazosin prevents the decrease of bNGF in myocardium of rats with heart failure.