全反式维甲酸对人胰腺癌细胞的抗增殖及诱导分化的实验研究

The proliferation inhibition and differentiation inducing effects of all trans retinoic acid on human pancreatic adenocarcinoma cell line JF 305

作者采用四甲基谷氮唑盐（ＭＴＴ）实验检测全反式维甲酸（ＡＴＲＡ）对人胰腺癌细胞系ＪＦ－３０５细胞的生长抑制作用；通过细胞病理学和电镜观察经ＡＴＲＡ作用后人胰腺癌细胞形态、超微结构的改变。结果提示：ＡＴＲＡ能够明显地抑制人胰腺癌细胞生长（Ｐ＜０．０５），随药物浓度增加而抑制作用增强，最大抑制率达３４．７％。经ＡＴＲＡ处理后增殖细胞明显减少（Ｐ＜０．０１），细胞内代谢减慢，细胞的分泌功能恢复，细胞恶性程度降低。作者认为：ＡＴＲＡ可抑制人胰腺癌细胞系ＪＦ－３０５细胞的生长并诱导其成熟分化。

We detected the antiproliferative effect with MTT test and investigated the changes in biological properties,cytomorphology and ultrastructure through cytopathology and electronic microscopy.Cell growth of JF 305 was inhibited by all trans retinoic acid(ATRA). The maximal inhibitory rate was 34 7%. The number of proliferative cells reduced( P <0 01). Cell metabolism slowed down, secretory functions recovered, and malignant degree decreased.ATRA can inhibit the proliferation and induce the differentiation of human pancreatic adenocarcinoma JF 305 cells.