人脑原发性肿瘤中p53基因的PCR-SSCP分析

PCR SSCP Analysis of p53 Gene in Human Primary Brain Tumor

为探讨ｐ５３基因突变与人脑原发性肿瘤发生的关系，采用ＰＣＲ－ＳＳＣＰ分析法，对３７例人脑原发性肿瘤中ｐ５３基因外显子５到外显子７之间的ＤＮＡ序列进行了突变分析。结果发现有１０例存在突变，其中９例为胶质瘤（３７．５％），１例为脑膜瘤（７．７％），且肿瘤恶性程度越高突变频率越高。以上结果表明，人脑原发性肿瘤的发生与ｐ５３基因的突变密切相关，ｐ５３基因突变有可能是人脑原发性肿瘤尤其是胶质瘤发生的原因之一。

In this study PCR SSCP alalysis was made to detect the mutation of the exon 5 7 of p53 gene in 37 cases of human primary brain tumor. The results showed that 10 human primary brain tumors had mutation, of which 9 were gliomas (37.5%) and 1 was meningioma (7.7%), and that the higher the grade of tumor was, the higher would be the frequency of mutation. These results suggest that human primary brain tumor is related to p53 gene mutation and such mutation may be the major reason for human primary brain tumor genesis and malignancy.