人血清载脂蛋白B100免疫浊度测定法研究

Quantitation of Human Apolipoprotein B100 by Immunoturbidimetric Assay

为寻求一种简易、快速、成批地测定人血清载脂蛋白Ｂ１００（ａｐｏＢ１００）的方法，作者采用自制羊抗人ａｐｏＢ１００血清，以免疫浊度测定法测定人血清ａｐｏＢ１００。此法灵敏、特异、易自动化操作，最小检测量为３．１μｇ，标准曲线测定范围为０．３４～１．７０ｇ／Ｌ，批内及批间重复变异系数分别为１．１３％～３．４８％及２．９１％～４．４８％。测定１００例正常成人血清ａｐｏＢ１００的含量（ｘ±ｓ）为０．８２１±０．１６５ｇ／Ｌ。文内还对影响测定的有关因素进行了讨论。

An immunoturbidimetric assay(ITA) for quantification of human serum apolipoprotein B100(apoB100)was developed.The assay is sensitive,rapid,specific,easily automatic and thus convenient for routine work.The minimu measurable concentration of apoB100 was 3.1μg in each assay.The standard curve with a working range of 0.34 1.79g/L was plotted.The between and within assay coefficients of variation were 1.13% 3.48% and 2.91% 4.48% respectively.The recovery was 99.23%±3.57%.The mean serum concentration of apoB100 in 100 normal subjects was 0.821±0.165g/L.The correlation coefficient of RID for apoB100 to ITA was 0.8626( P <0 001)