明矾处理对雨生红球藻形态结构的影响

Effects of alum on the morphological structure of Haematococcus pluvialis

试验用显微镜和透射电镜观察了不同明矾浓度处理后的雨生红球藻(Haematococcus plu-vialis)的显微和超微结构变化。试验以MAV培养基为基本培养基,在温度为20±1℃、光强为40μmol/m2s条件下进行扩增培养,达到一定量后用不同浓度的明矾进行处理。浓度梯度分别为0、20、40、60、80、100、120、140mg/L,暗处理24h后计算其沉降率,用显微镜和透射电镜观察并拍照,比较其显微与超微形态结构上的差异。结果表明,随着明矾浓度的升高,藻的沉降率逐步上升,明矾可以快速且有效地使雨生红球藻凝集并沉淀,浓度为60mg/L的明矾对雨生红球藻不会造成伤害,但会延迟和影响虾青素的积累。

The microstructure and uhrastructure of Haematococcus pluvialis cells dis posed to different alum concentrations were observed with microscope and transmission electron microscope. Haematococcus pluvialis was cultivated in the MAV medium at 20± ℃ and 40 μmol/m^2s. After reaching a certain density, Haematococcus pluvialis was exposed to different concentrations of alum, viz. 0, 20, 40, 60, 80, 100, 120, and 140 mg/L, in dark for 24 h. Then sedimentation rates were calculated, Haematococcus pluvialis cells were observed and taken picture with microscope and transmission electron microscope, aiming at finding the changes in microstructure and ultrastructure. The results showed that the sedimentation rates of Haernatococcus pluvialis rose along with increasing of alum concentrations, indicating that Alum could make H. pluvialis cells gather together and deposit effectively. Besides, alum at the concentration of 60× 10^-6 did not produce harmful effect on the structure of the alga cells, but it would retard and affect the accumulation of astaxanthin.