用SSR标记分析抗疫霉根腐病大豆品种(系)的遗传多样性

Genetic Diversity of Soybean Cultivars (Lines) Resistant to Phytophthora sojae Based on SSR Markers

利用50对SSR引物对抗疫霉根腐病大豆品种(系)进行遗传多样性分析。在166份品种(系)中,50个SSR座位共产生265个等位变异,平均每个座位5.3个。采用NTSYS-pc2.10计算品种(系)间遗传相似系数,平均相似系数为0.3124,表明抗疫霉根腐病大豆品种(系)间的遗传差异较大。用UPMGA进行聚类分析,166个品种(系)在相似系数为0.33时被聚为6类,地理来源相同的品种(系)大多聚类在一起。一些具有相同或相近抗病反应型的品种(系)被聚类在同一个类群中,表明这些抗病品种(系)的遗传关系较近,应有选择地利用。W illiam s和C lark抗疫霉根腐病近等基因系构成明显不同于中国大豆的基因源,可以用于拓宽我国大豆品种的遗传基础。

Using 50 SSR makers to analysis genetic diversity among Phytophthora resistant soybean cuhivars （lines） , 265 allelic variants were detected among the cultivars （lines） with average 5.3 alleles per SSR locus. The average genetic similarity coefficient 0. 3124, indicating that significant genetic difference existed among the cultivars （lines）. Clustering analysis showed when the similarity coefficient was 0.33 the cultivars （lines） were divided into six groups, and the cuhivars or lines with the same geographical origin were clustered together. Those cultivars or lines with the same or similar resistance type to Phytophthora sojae were clustered into the same group, it indicated that they should have closer genetic relationship, and should be selectively used. Near-isogenic lines of cuhivars Williams and Clark with single Rps gene were different from Chinese Phytophthora resistant soybean cuhivars or lines, and could be used to widen the genetic background of Chinese soybean cuhivars.