热休克蛋白在肺腺癌细胞及组织中的表达

Expression profile of heat shock proteins in tissues and cells of lung adenocarcinoma

目的:探讨热休克蛋白(heat shock proteins,HSPs)在肺腺癌A549细胞及组织中的表达情况。方法:分别以正常人支气管上皮(human bronchial epithelium,HBE)细胞和正常肺组织为对照,采用免疫印迹及RT-PCR的方法,从细胞水平和组织水平观察HSP86,HSP70及aB晶状体蛋白在肺腺癌A549细胞及组织中的表达情况。结果:与HBE细胞及正常肺组织比较,A549细胞及癌组织中HSP86,HSP70及aB晶状体蛋白的mRNA与蛋白质表达均显著增加(P<0.05),其中以HSP70的增加最为显著(P<0.01)。结论:肺腺癌细胞及组织中HSPs的表达量高于HBE细胞及正常肺组织,其中以HSP70的增加最为显著。

Objective To observe the expression profile of heat shock proteins （ HSPs ） including HSP70, inducible HSP90 （HSP86） and aB-crystallin in cells and tissues of lung adenocarcinoma. Methods Western blotting and reverse transcriptional-polymerase chain reaction （RT-PCR） were performed to detect the expression of HSP70, HSP86 and aB-crystallin both in the protein and mRNA level respectively. Results Compared with normal lung tissue and human bronchial epithelium （HBE） cells, RT-PCR and Western blotting showed that the expression of HSP70, HSP86 and αB crystallin increased significantly in both the mRNA and protein level in the cancer tissue and A549 human lung adenocarcinoma cells. Among the 3 sub-families of HSPs, the expression of HSP70 mRNA and protein increased most in both the lung tissue of cancer and A549 human adenocarcinoma cell lines. Conclusion The expression of HSPs is higher in the lung adenocarcinoma and A549 cells than that in the normal lung tissues and HBE cells. Among the HSP family, HSP70 is the most up-regulated member in the tissue and cells of lung adenocarcinoma.