慢性胃炎中医湿证血清蛋白组学研究

Serum proteomics study of chronic gastritis with dampness syndrome in traditional Chinese medicine

目的:对慢性胃炎湿证患者血清蛋白组学进行分析,并探讨慢性胃炎湿证的微观信息。方法:采用表面增强激光解吸离子化蛋白质芯片分析仪对18例慢性胃炎湿证、17例非湿证患者及8例正常人血清蛋白质表达谱进行研究。结果:慢性胃炎湿证患者蛋白质表达谱质荷比为3.2 kD、6.4 kD、8.1 kD,出现高表达趋势,其波峰基本在10个单位以上;非湿证患者与正常人上述波谱峰呈低表达趋势,非湿证患者波峰基本在10个单位左右或以下;正常人波峰基本在5个单位以下。结论:血清蛋白组学分析对探索慢性胃炎湿证的生物学理论基础有一定意义。

Objective： To explore microcosmic information in chronic gastritis dampness syndrome by using serum proteomics of patients with chronic gastritis dampness syndrome. Methods： Serum proteomics of 18 dampness syndrome cases, 17 non-dampness syndrome cases in chronic gastritis patients and 8 normal controls were analyzed by surface enhanced laser desorption/ionization-time of flight （SELDI-TOF） protein-chip. Results： There was a high expression trend in three ratios of charge （of an electron） to mass （M/S） of 3.2 kD, 6.4 kD and 8. 1 kD of protein expression spectrum from patients with chronic gastritis dampness syndrome, and the wave peak value was over 10 units. There was a low expression trend in above-mentioned sites of protein expression spectrum from patients with chronic gastritis non-dampness syndrome and normal group. The wave peak value of patients with chronic gastritis non-dampness syndrome was about or below 10 units, and the wave peak value in normal group was below 5 units. Conclusion： There exists a certain significance to explore biologic theory basis of chronic gastritis dampness syndrome by analyzing the serum proteomics.