雷公藤化合物对鸡胚绒毛尿囊膜血管生成的影响

Effects of three compounds extracted from Tripterygium wilfordii Hook on angiogenesis in chick chorioallantoic membrane

目的:通过观察三种雷公藤(Tripterygium wilfordii Hook,TW)化合物TW1、TW2和TW3对鸡胚绒毛尿囊膜(chick chorioallantoic membrane,CAM)血管生成的影响,探讨雷公藤的抗新生血管形成作用。方法:50枚新鲜香港麻花鸡种蛋,随机分为5组:PBS组、TW1组、TW2组、TW3组、参一胶囊(ginsenoside,Rg3)组,每组各10枚。种蛋消毒后,放入37.8C恒温箱内孵育6 d,暴露尿囊膜,将含有待测品的滤纸放在尿囊膜血管较少部位,继续孵育2 d,以滤纸为中心,把膜剪下,固定,拍照并记录各组CAM血管生长情况。结果:PBS组CAM血管及其网络清晰可见,生长良好;TW1、TW2、TW3(剂量均为10μg/枚)处理组的毛细血管数目较PBS组明显减少,血管结构模糊,颜色变浅、苍白,三者对CAM血管生成的抑制率分别为80%、60%、100%;阳性对照Rg3在相同剂量时,对CAM血管生成的抑制率仅10%。结论:三种雷公藤化合物TW1、TW2、TW3对CAM血管生成均有明显的抑制作用,值得进一步研究。

Objective. To investigate the effects of three compounds extracted from Tripterygium wilfordii Hook （TW） on angiogenesis in the chick chorioallantoic membrane （CAM）. Methods： Fifty fresh Hongkong Mahua chicken eggs were divided into five groups： PBS-treated group, TW1-, TW2- and TW3-treated groups and Rg3-treated group. After disinfection, the eggs were incubated for six days in a constant temperature box with the temperature being controlled within 37.8℃, then exposed CAM, laid the filter papers with specimen on the CAM, and the eggs were incubated for another two days, CAM was fixed with the mixture of methyl alcohol and acetone at room temperature for about 15 min, and then cutting the CAM, taking photos and observing the angiogenesis in the CAM, Results： There were many CAM vessels in the PBS-treated group and the blood vessel net could be seen clearly. The number of CAM vessels in the TW1-, TW2- and TW3-treated groups （10 fig/egg） was much less than that in the PBS-treated group, Furthermore, the frame of the vessels was not clear, and the color was obscure, Inhibition rates of angiogenesis in the TW1-, TW2- and TW3-treated groups were 80%, 60% and 100% respectively, while the inhi- bition rate of angiogenesis in the Rg3-treated group （10 fig/egg） was only 10%. Conclusion： TW1, TW2 and TW3 can obviously restrain the angiogenesis in CAM and still need further study,