温阳除痹汤对硬皮病模型小鼠皮肤结缔组织生长因子及Ⅰ型胶原蛋白表达的影响

Effects of Wenyang Chubi Decoction on connective tissue growth factor and collagen-Ⅰin a mouse model of scleroderma

目的:研究温阳除痹汤对硬皮病小鼠结缔组织生长因子(connective tissue growth factor,CTGF)、Ⅰ型胶原蛋白(collagen-Ⅰ,COL-Ⅰ)表达的影响。方法:将博来霉素诱导的硬皮病小鼠随机分为3组:模型组、生理盐水组和温阳除痹汤组,以正常BALB/c小鼠作为对照。生理盐水组和温阳除痹汤组分别灌服生理盐水和温阳除痹汤,连续给药1个月。观察皮肤和肺组织病理学变化,并采用免疫组化技术检测各组硬皮病小鼠皮损中CTGF和COL-Ⅰ蛋白含量。结果:造模组小鼠皮肤CTGF和COL-Ⅰ免疫组化指数较对照组明显增加,差异有统计学意义(P<0.05)。与灌服生理盐水的模型小鼠比较,温阳除痹汤组小鼠皮肤和肺组织学有明显改善(P<0.05);温阳除痹汤组CTGF及COL-Ⅰ蛋白含量较生理盐水组亦显著降低,差异有统计学意义(P<0.05)。结论:CTGF及COL-1在硬皮病小鼠皮肤组织中具有较高表达,温阳除痹汤可明显降低CTGF、COL-Ⅰ含量,改善皮肤硬化。

Objective： To study the effects of Wenyang Chubi Decoction （WYCBD）, a compound traditional Chinese herbal medicine, on connective tissue growth factor （CTGF） and collagen-Ⅰ （COL-Ⅰ ） in a mouse model of scleroderma. Methods： Scleroderma was induced in BALB/c mice by daily local injection of bleomycin for three weeks and the mice were randomly divided into untreated, WYCBD-treated and normal saline （NS） treated groups, with another group of BALB/c mice as normal control. WYCBD and NS were given orally for one month respectively. Histopathology in the skin and lungs of the mice were examined. The CTGF and COL-Ⅰ expressions in the skin or skin lesions were detected by immunohistochemical Elivision assay. Results ： The expression levels of CTGF and COL- Ⅰ in the untreated group were significantly higher than those in the normal control group （P〈0.05）. Compared with the NS-treated group, the WYCBD-treated group had significant improvement in the skin and lung histopathology and remarkably decreased expression levels of CTGF and COL- Ⅰ （P〈0. 05）. Conclusion： Scleroderma mice showed high expressions of CTGF and COL-Ⅰ in the skin. WYCBD had the effects of decreasing the CTGF and COL- Ⅰ expressions and improving the skin fibrosis.