ERF转录因子StERF1的生物信息学分析

Bioinformatical Analysis of ERF Transcription Factor StERF1

从生物信息资源中寻找有重要功能的新ERF基因,命名为StERF1,用生物信息学方法对其主要理化性质和功能进行分析预测,为下一步的试验策略提供参考。StERF1编码一个由247个氨基酸组成的相对分子质量为27203.9的蛋白质,该蛋白具有一个由58个氨基酸残基组成的保守ERF结构域(112～169),在N端有一个可能起激活作用的酸性结构域,在C端有一个可能作为核定位信号(NLS)的碱性结构域,它可以结合GCC-box顺式作用元件,推测StERF1是一个定位在核内发挥转录激活作用的ERF转录因子。它可能在调控植物乙烯信号途径、生物胁迫或非生物胁迫应答过程发挥重要作用。

An important functional novel ERF gene was found from intemet information resource and named StERF1. The physical and chemical characteristics of this novel gene as well as its secondary and tertiary structures and function was analyzed and predicted by using bioinformatical methods. The StERF1 encodes a putative protein containing 247 amino acid with a predicted molecular mass of 27.2 ku and contains a conserved ERF DNA binding domain （112-169）. Database analysis suggested that the deduced amino acid sequence of TSRF1 contain a basic region that might function as a nuclear localization signal （NLS） and an acidic region that might act as transcriptional activation domain. This suggests that StERF1 is an ERF transcription factor which can activate the expression of GCC-box localized in the nuclear, and that StERF1 may regulate the plant ethylene response pathway, biotic stress and abiotic stress response.