脂肪来源细胞和透明质酸钠复合物构建裸鼠皮下软组织

Engineered subcutaneous soft tissue constructed from human adipose-derived cells and sodium hyaluronate in nude mice

目的探讨脂肪来源细胞(ADCs)作为新型真皮充填物的可能性。方法ADCs取自3例行整复手术的男性患者的残余脂肪组织,支架材料为透明质酸钠。实验分为三组,实验组:ADCs(25×106/mL)与透明质酸钠混合,每一样本0.2mL注射于雌性BLAB/c裸鼠皮下;对照组1:单纯运用透明质酸钠,每一样本0.2mL注射于裸鼠皮下;对照组2:单纯运用ADCs(25×106/mL),每一样本0.2mL注射于裸鼠皮下。8周后取材,通过大体和组织学观察,以及性染色体RT-PCR检测,对再生组织进行评价。结果实验组形成软组织呈球形,平均体积(0.0352±0.0115)mL;组织学观察显示,生物材料完全降解,组织排列紊乱,间有大量排列紊乱的胶原纤维,无脂肪组织;两对照组均无任何组织形成。结论应用组织工程技术,以人脂肪来源细胞为种子细胞,透明质酸钠为支架材料,能构建出良好的组织工程化软组织;为探索一种安全、有效的除皱方法,提供了新的研究方向。

Objective To study the feasibilility of engineered subcutaneous soft tissue constructed from human adipose derived cells （ADCs）. Methods ADCs were harvested from the remnant adipose tissue of 3 male patients with plastic and reconstructive surgery, and sodium hyaluronate was served as scaffold material. Three groups were divided for the experiment. In the experiment group, ADCs （25 ×10^6/mL）were mixed with sodium hyaluronate gel, and were subcutaneously injected at 0. 2 mL per implant into the back of a female BLAB/c nude mouse. Sodium hyaluronate gel without ADCs （control group 1 ） and ADCs （25 ×10^6/mL）without scaffold （control group 2） were subcutaneously injected at 0. 2 mL per implant into the back of nude mouse. Implants were harvested after 8 weeks of in vivo culture, and then were measured with volume, examined with histological staining and detected with human Y chromosome by RT- PCR. Results The mean volume of engineered nodules from the experiment group was （0, 0352 ±0. 0115）mL. Histologically, the engineered nodules had collagen in disorder and no adipose. In the two control groups, no nodule was formed. Conclusion The complex of ADCs as seed cells and sodium hyaluronate gel as scaffold can form better engineered soft tissue, which paves a safe and effective way for dermal and subcutaneous defects repair.