相对定量的2^(-△△CT)法分析HLA-G基因在子宫内膜异位症中的表达

Relative quantitative detection of HLA-G mRNA expression in patients with endometriosis by 2^(-△△CT) Method

目的检测人类白细胞抗原-G(HLA-G)在子宫内膜异位症(EM)在位与异位内膜组织中的表达。方法选取卵巢子宫内膜异位囊肿患者30例(实验组),非子宫内膜异位症患者30例(对照组),应用实时荧光定量RT-PCR技术检测实验组在位与异位内膜组织,及对照组在位子宫内膜组织中HLA-G mRNA的表达,2-△△CT法分析结果。结果HLA-G mRNA在实验组在位与异位内膜组织中的表达均明显高于对照组(P<0.05),两者比值约为3.5/1。实验组在位与异位内膜组织中的表达无明显差异(P>0.05)。结论HLA-G mRNA的表达异常可能与EM发病有关。

Objective： To investigate Human leukocyte antigen- G （HLA- G） mRNA expression in the endometrium of endometfiosis （EM）. Methods： 30 women with ovarian endometriosis （OEM）, 30 women without EM were recruited as study grou and control group, respectively. FQ - RT PCR was used for quantitative detection of the difference on HLA - G mRNA expression in the endometrium between two groups. 2^-△△CT method was used for analyzing the difference on HLA - G mRNA expression. Results： HLA - G mRNA expression of OEM group was significantly higher than that in the control group, the ratio is 3. 5/1. There was no obvious correlation between the expression of OEM and adenomycsis （AM）. Conclusion： Higher HLA - G mRNA may be related to the pathomechanism of EM.