益气活血方对大鼠颈总动脉球囊损伤后血管壁的影响

Effects of the Yiqi Huoxue Prescription on Carotid Arteries of Balloon-Injured Rats

目的:观察益气活血方对大鼠颈总动脉球囊损伤后血管壁的影响。方法:SD大鼠48只,随机分成4组。用2.0F球囊导管自右股动脉插管建立大鼠左颈总动脉球囊损伤模型,治疗结束,麻醉,心脏放血处死大鼠,取损伤大鼠左颈总动脉,常规固定、包埋、切片,做HE染色和Masson染色。用HMIAS-2000型全自动彩色图象分析系统,观察动脉球囊损伤后不同时相点血管壁病理改变,进行比较分析。结论:成功复制球囊损伤术后颈总动脉再狭窄动物模型;益气活血方可显著防止或减轻大鼠颈总动脉球囊扩张后所致的血管狭窄,益气活血方大剂量作用更明显。

Objective ： In order to observe the influence of the yiqihuoxue prescriptions therapy on carotid arteries of ballooninjured rats. Methods ：48male Sprague-Dawley big rats were randomly divided into 4 groups （ n = 6）. The 2. OF balloon catheter injury was perfomed through the right femoral artery into the left common carotid artery of rats. Around 6 rats from each group were euthanized by exsanguination at appropriate time point. The injuried left common carotid arteries were carefully removed and harvested for HE staining and Massons staining. Microscopic analyses and quantitation of morphologic parameters were performed using HMIAS - 2000, which is a full - automatic computerized colorful imaging analysis system. The system analysis beteen the each correlation index of RS and Chinese herbal medicine were performed as well. Results ：This experiment successfully duplicated the clinical animal model of RS, the model method had the worthy of expansion. Experiment study revealed that the yiqihuoxue prescriptions could prevent and reduce the rate of restenosis after rat carotid balloon injury,they might be a potential therapeutic approach to preventing restenosis. Furthermore, the effect of the high - dose yiqihuoxue prescription group were remarkable than that of the low - dose yiqihuoxue prescription group.