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RT-PCR法克隆拟南芥中NPR1基因及其植物表达载体的构建 被引量：1

Amplified by RT-PCR and plant expressing vector construction of the Arabidopsis thaliana NPR1 disease-resistance gene
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摘要 园林植物多进行保护地栽培,高温高湿的局部小气候使园林植物更容易感染多种病害,一旦染病则极大地影响园林植物的观赏价值,造成较大的经济损失。文章采用RT-PCR扩增的方法从拟南芥基因组中克隆到能够介导植物广谱抗病的NPR1基因,并将其构建植物表达载体,为下一步的转化园林植物工作打下基础。 The horticultural plants usually are cultivated in the greenroom where is easy to cause many bacterial and fungal diseases. Once they were infected by all kinds of diseases, the horticultural plants＇ value of view and admire was seriously affected and the economy value was seriously reduced. In the experiment, NPR1 gene which can significantly enhance the resistance for broadrange of pathogens from the Arabidopsis thaliana genome was amplified by RT-PCR and the plant expression vector containing NPR1 gene was constructed. It provides an avenue for transgenic breeding of horticultural plants.
作者 刘永光 车代弟 王金刚 龚束芳 樊金萍
机构地区 东北农业大学园艺学院
出处 《东北农业大学学报》 CAS CSCD 2007年第4期491-494,共4页 Journal of Northeast Agricultural University
基金 黑龙江省自然科学基金(C2004-01) 黑龙江省研究生创新科研专项基金(YJSCX2005-200HLP)
关键词 NPR1基因 广谱抗病 载体构建 NPRI gene disease-resistance for broad range of pathogens construction of plant expression vector
分类号 Q786 [生物学—分子生物学]
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参考文献10

  • 1张红志,蔡新忠.病程相关基因非表达子1(NPR1):植物抗病信号网络中的关键节点[J].生物工程学报,2005,21(4):511-515. 被引量：42
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二级参考文献36

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东北农业大学学报
2007年 第4期

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