摘要
目的采用高效液相色谱法测定炎可宁片中盐酸小檗碱的含量。方法Kromasil C18色谱柱(250 mm×4.6 mm,5μm);乙腈-0.05 mol·L^-1磷酸二氢钾溶液(30∶70)为流动相,流速为1.0 ml·min^-1;检测波长为347 nm。结果盐酸小檗碱线性范围为20.2-181.8 mg·L^-1(r=0.999 9,n=5),平均回收率为99.71%,RSD=0.32%(n=6)。结论该方法准确、简便,可用于该制剂的质量控制。
Aim To establish a HPLC method for determining berberine in Yankening tablets. Methods C18 column (250 mm × 4.6 ram,5μm) was used and the mobile phase was acetonitrile-0. 05 ·min^-1 KDP (30: 70). The flow rate was 1.0 ml ·min^-1 . Diode array detector was set at 347 nm. Results The linear range of berberine was between 20.2 and 181.8 nag · L ^- 1 ( r = 0. 999 9,n = 5 ) and the average recovery were 99.71% with RSD being 0, 32% ( n=6 ). Conclusion The method is accurate and simple for quality control of the preparation. Key words : HPLC ; berberJne ; yankening tablets
出处
《安徽医药》
CAS
2007年第9期790-791,共2页
Anhui Medical and Pharmaceutical Journal