转Bt+Sck棉花的分子检测及其农艺性状分析

Molecular detection and analysis of agronomic traits of transgenic cotton lines with Bt+Sck genes

以苏棉16为受体通过花粉管通道法导入Bt+Sck基因获得5个转基因株系(301-5 T2、305-5 T2、312-5 T2、314-4T2和332-2 T2),对这5个转基因株系进行了分子杂交及其农艺性状评价。与受体苏棉16相比较,转基因株系的单株铃数和抗虫性显著提高;叶型变小、叶色加深;株高变化不大;铃重、衣分降低,其中305-5 T2和314-4 T2的衣分显著低于受体苏棉16;转Bt+Sck基因纯系的纤维品质与受体苏棉16无显著性差异;除305-5 T2产量低于受体苏棉16外,其余纯系与受体苏棉16的皮棉产量持平。在相同的遗传背景下,不同的转Bt+Sck基因纯系对棉花农艺性状表现所产生的效应有所不同,为培育新的品种提供了可能。

The molecular hybridization and agronomic traits of five homologous lines （301-5 T2, 305-5 T2, 312-5 T2, 314- 4 T2 and 332-2 T2 ）, which obtained from Sumian 16 transformed with Bt and Sck genes by the pollen-tube pathway-mediated transformation method, were analyzed, Compared with Sumian 16, bolls per plant and resistance to bollworm of these five transgenic homologous lines increased, leaf became smaller, leaf color deepened, boll size and lint percentage decreased, the lint percentage of 305-5 T2 and 314-4 T2 was significantly lower than that of Sumian 16, The plant height had no significant difference with that of Sumian 16. The fiber quality had no significant difference among these homologous transgenic lines and Sumian 16, The lint yields of all the transgenic lines except for 305 - 5 T2 were no difference with Sumian 16 plants. The important agronomic traits of these five homologous lines were different at the same genetic background, The new transgenic cotton varieties with high efficiency to cotton bollworm would be developed using these five homologous lines,