香烟烟雾对大鼠晶状体脂质过氧化与抗氧化作用的影响

Effect of Cigarette Smoke on Lipid Peroxidation and Antioxidation in Rat Lens

目的观察香烟烟雾对大鼠晶状体的脂质过氧化作用以及对抗氧化酶活性和非蛋白质巯基的影响。方法将大鼠造成香烟烟雾损伤模型并测定晶状体中脂质过氧化产物丙二醛（ｍａｌｏｎｄｉａｌｄｅｈｙｄｅ，ＭＤＡ）和非蛋白质巯基（ｎｏｎ－ｐｒｏｔｅｉｎｓｕｌｐｈｙｄｒｙｌｇｒｏｕｐ，ＮＰ－ＳＨ）的水平以及超氧化物歧化酶（ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ，ＳＯＤ）和谷胱甘肽过氧化物酶（ｇｌｕ－ｔａｔｈｉｏｎｅｐｅｒｏｘｉｄａｓｅ，ＧＳＨ－Ｐｘ）的活性。结果吸烟组大鼠晶状体中ＭＤＡ含量明显增加（Ｐ＜０．０１），ＮＰ－ＳＨ的含量、ＳＯＤ和ＧＳＨ－Ｐｘ的活性均显著降低（Ｐ＜０．０１）。结论香烟烟雾可能是导致白内障形成的重要原因之一。

ObjectiveTo observe the effect of cigarette smoke on the lipid peroxidation,the activity of antioxidases,and the level of non protein sulphydryl group in the rat lenses.MethodsWe determined the levels of MDA and NP SH,and the activity of SOD and GSH Px in the lenses of rat injured by cigarette smoke.ResultsIn the smoking rats MDA content was higher,the concentration of NP SH,the activity of SOD and GSH Px were decreased. In comparison with control group,there were significant differences ( P <0.01).ConclusionCigarette smoke may be involved in the development of cataract.