氟伐他汀对糖尿病大鼠肾脏核因子κB表达的影响

Effects of Fluvastatin on the Expression Nuclear Factor-kappa B in the Kidney of Diabetic Rats

目的:通过糖尿病大鼠模型,观察氟伐他汀对大鼠肾脏的保护作用。方法:SD大鼠随机分3组,即正常对照组、糖尿病组(DM组)、氟伐他汀治疗组。以链脲佐菌素(STZ)制备糖尿病大鼠模型,大鼠饲养4周分别取出肾脏检测NF-κB的表达。检测各组大鼠血糖、血脂2、4 h尿蛋白定量等指标。采用免疫组织化学技术检测各组肾小球中NF-κB的表达。结果:糖尿病大鼠肾小球NF-κB表达较正常肾组织增高,治疗4周后,小剂量氟伐他汀对血糖、血脂水平无影响,但可明显降低糖尿病肾病大鼠肾小球中NF-κB的表达,并减少蛋白尿。结论:氟伐他汀对糖尿病大鼠肾脏保护作用可能通过非依赖降脂的保护作用,其机制至少部分与下调肾小球中NF-κB表达有关。

Objective： To study the protective effects of fluvastatin on the kidneys of diabetic rats. Methods： The models of diabetic rats were induced by streptozotocin. SD rats were randomly divided into three groups as control group, diabetic group and fluvastatin treated group. After four weeks, blood glucose, serum cholesterol and urinary protein were measured respectively. The expression of NF-κB in the glomerulus was determined by immunohistochemical staining. Results： Fluvastatin did not influence blood glucose and serum cholesterol, but significantly inhibited the expression of NF-κB and lowered urinary protein excretion. Conclusion： Fluvastatin could protect the kidneys of diabetic rats probably through inhibiting the overexpression of NF-κB,independent of cholesterol-lowering effects.