高糖对血管内皮细胞凋亡的影响及卡托普利的干预作用

The protective effects of captopril on apoptosis of human umbilical vein endothelial cells under high concentrations of glucose in vitro

人脐静脉内皮细胞培养分5组:正常葡萄糖为对照,高糖浓度有20mmol/L,40mmol/L,20mmol/L糖联合10-5mol/L卡托普利,40mmol/L糖联合10-5mol/L卡托普利。细胞凋亡和Fas、Bcl-2基因表达均用流式细胞仪测定。高糖组均引起细胞凋亡增加,引起Fas表达增加和Bcl-2表达减少。卡托普利能够减少高糖所致细胞凋亡,减少Fas表达,增加Bcl-2表达。

The human umbilical vein endothelial cells（HUVEC） were cultured in five groups：NG（Glu 5.5 mmol/L,normal control）,HG20（Glu 20 mmol/L）,HG40（Glu 40 mmol/L）,HG20-Cap（Glu 20 mmol/L,Cap10-5 mol/L） and HG40-Cap（Glu 40 mmol/L,Cap10-5 mol/L）.Apoptosis of the cells and the expressions of Fas,Bcl-2 were determined and evaluated by flow cytometry.Exposure to HG resulted in an increase in apoptosis in both HG20 and HG40 groups（P〈0.01）.HG enhanced the expression of Fas and decreased Bcl-2 expression.Captopril reduced high-glucose-induced HUVEC apoptosis accompanied by a decrease of Fas expression and an increase of Bcl-2 expression.