系统性红斑狼疮患者高表达的CD154促进自身抗体分泌被引量：1

Increased CD154 Expression on Peripheral Blood Mononuclear Cells Stimulates Secretion of Autoantibodies in Patients with Systemic Lupus Erythematosus

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摘要【目的】探讨系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)CD154表达水平、及CD40/CD154对SLE患者分泌自身抗体的影响和地塞米松(Dex)对此的作用。【方法】选择SLE活动期患者(10例)和正常对照者(11例),分离PBMC,检测其CD154的表达水平;培养PBMC,应用CD40 mAb和Dex进行干预,使用ELISA法检测培养液上清抗dsDNA水平。【结果】①活动期组PBMC CD154表达水平明显高于对照组6.4%±2.1%vs 1.5%±1.2%,(P<0.05);②活动期组培养液上清抗dsDNA抗体水平明显高于对照组[(11.8±6.8)U/mL vs(5.6±2.3)U/mL(RPMI 1640),(11.4±7.0)U/mL vs(6.3±2.1)U/mL(IgG),P均<0.05];③CD40 mAb使两组培养液上清抗dsDNA抗体水平明显增高[活动期组:(18.6±7.7)U/mL vs(11.8±6.9)U/mL,对照组:(8.3±4.4)U/mL sv(5.6±2.3)U/mL,P均<0.05]。④在活动期组,Dex明显抑制CD40 mAb引起的PBMC培养液上清抗dsDNA抗体水平的增高[(8.8±5.2)U/mL vs(18.6±7.7)U/mL,P<0.05],使其低于非特异性处理时水平[(8.8±5.2)U/mL vs(11.8±6.8)U/mL,P<0.05];Dex不影响对照组。【结论】活动期SLE患者PBMC表达的CD154水平升高,此能够促进抗dsDNA抗体的分泌,而Dex能够抑制其作用。 [Objective ] To investigate the expression of CD154 on peripheral blood mononuclear cell （PBMC） and the effects to secretion of autoantibodies by CD154 and dexamethasone in the patients with systemic lupus erythematosus （SLE）. [Methods] The PBMC from active SLE patients and healthy control were isolated and sent to measure expression of CD154 by flow cytometry, the left PBMC were cultured and treated with CD40 mAb or Dex. The supernatant concentration of anti-dsDNA antibody was measured by ELISA. [Results] （1） The expression of CD 154 on PBMC （ % ） in active SLE were higher than that in healthy control （6.4±2.1 vs 1.5±1.2, P〈 0.05）. （2） The supernatant concentration of anti-dsDNA antibody in active SLE were higher than that in healthy control [11.8± 6.8 vs 5.6±2.3 （RPMI 1640）, 11.4±7.0 vs 6.3±2.1 （IgG） U/mL, all P〈 0.05]. （3） CD40 mAb could significantly increase the supernatant concentration of anti-dsDNA antibody in the two groups [18.6±7.7 vs 11.8±6.9 （active SLE）, 8.3±4.4 vs 5.6±2.3 （control） U/mL all P〈 0.05）. （4） Dexamethasone could decrease the elevated supernatant concentration of anti-dsDNA caused by CD40 mAb in active SLE （ 8.8±5.2 vs 11.8±6.8 U/mL P〈 0.05 ） to a lower level （ 8.8±5.2 vs 11.8±6.8 U/mL P〈 0.05）, but no effects to healthy control. [Conclusion] The expression of CD154 on PBMC were increase and could stimulate the secretion of anti-dsDNA in active SLE, and this effect were inhibited by Dex.

作者李玉杰李幼姬张涤华许元文董秀清

机构地区中山大学附属第一医院急诊科中山大学附属第一医院肾内科

出处《中山大学学报（医学科学版）》 CAS CSCD 北大核心 2007年第5期511-514,共4页 Journal of Sun Yat-Sen University:Medical Sciences

基金广东省医学科学研究基金(A2001151) 广东省自然科学基金(04300354)

关键词红斑狼疮系统性自身抗体 CD154 地塞米松 lupus erythematosus, systemic autoantibody CD154 dexamethasone

分类号 R593.24 [医药卫生—内科学]

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参考文献14

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