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氯通道阻断剂对H_2O_2诱导的胰岛RIN-mβ细胞凋亡的影响 被引量：2

Effects of chloride channel blockers on H_2O_2 induced apoptosis in pancreas RIN-m beta cells
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摘要 目的观察氯通道阻断剂对H2O2诱导的胰岛RIN-mβ细胞凋亡的影响,探讨氯通道在RIN-mβ细胞凋亡中的作用。方法采用H2O2诱导的胰岛RIN-mβ细胞凋亡模型,观察氯通道阻断剂(DIDS、NPPB和NFA)对细胞存活率、形态学变化、凋亡的影响。结果氯通道阻断剂单用时对胰岛RIN-mβ细胞活力无明显影响,但能明显提高H2O2处理的胰岛RIN-mβ细胞的存活率。与模型对照组相比,氯通道阻断剂DIDS、NPPB和NFA对抗组细胞存活率明显增加(P<0.01),细胞凋亡率明显降低(P<0.01)。结论氯通道阻断剂对H2O2诱导的RIN-mβ细胞损伤和凋亡具有明显的保护作用。 　Aim To investigate the effects of chloride channel blockers on the apoptosis of RIN-mβ cells of pancreatic islet induced by H2O2.Methods The apoptotic model was made by H2O2 exposed for six hours with a concentration of 500 μmol·L^-1.The chloride channel blockers：DIDS,NPPB and NFA were administered to pretreat the samples respectively.The cell viability,morphological changes,and apoptosis rate were observed.Results Chloride channel blockers alone have no marked effects on the cell viability of RIN-mβ cell.However,they elevated the cell viability of RIN-mβcell disposed of by H2O2.Compared to H2O2 group,the groups of DIDS ＋H2O2,NPPB＋ H2O2 and NFA＋H2O2 have significant difference in cell viability and apoptosis rate（P〈0.01）.Conclusions Chloride channel blockers（DIDS,NPPB and NFA） remarkably inhibit apoptosis of RIN-mβ cells induced by H2O2.
作者 任省华 叶春玲 金永亮 吕艳青 冯娟
机构地区 暨南大学药学院药理学教研室
出处 《中国药理学通报》 CAS CSCD 北大核心 2007年第9期1202-1206,共5页 Chinese Pharmacological Bulletin
基金 广东省自然科学基金资助项目(No04010463)
关键词 胰岛RIN-mβ细胞 凋亡 过氧化氢 氯通道阻断剂 pancreas RIN-mβ cell,apoptosis,H2O2,chloride channel blocker
分类号 R322.57 [医药卫生—人体解剖和组织胚胎学] R329.24 [医药卫生—人体解剖和组织胚胎学]
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  • 1张海宁,关永源,丘钦英,贺华.反义ClC-3寡核苷酸促进thapsigargin诱导的PC12细胞凋亡[J].中国药理学通报,2004,20(6):614-619. 被引量：1
  • 2Skowasch D,Jabs A,Andrie R,et al.Pathogen burden,inflammation,proliferation and apoptosis in human in-stent restenosis.Tissue characteristics compared to primary atherosclerosis[J].J Vasc Res,2004,41 (6):525-34.
  • 3d'Anglemont A,Souktani R,Henry P,et al.Volume-sensitive chloride channels(ICl,vol) mediate doxorubicin-induced apoptosis through apoptotic volume decrease in cardiomyocytes[J].Fundam Clin Pharmcol,2004,18(5):531 -8.
  • 4Wei L,Xiao A Y,Jin C,et al.Effects of chloride and potassium channel blockers on apoptotic cell shrinkage and apoptosis in cortical neurons[J].Pflugers Arch,2004,448 (3):325-34.
  • 5Szabo I,Lepple-Wienhues A,Kaba KN,et al.Tyrosine kinasedependent activation of a chloride channel in CD-95-induced apoptosis in T lymphocytes[J].Proc Natl Acad Sci USA,1998,95(11):6169 -74.
  • 6Eggermont J,Trouet D,Carton I,et al.Cellular function and control of volume-regulated anion channels[J].Cell Biochem Biophys,2001,35(3):263 -74.
  • 7Duan D,Winter C,Crowley S,et al.Molecular identification of a volume-regulated chloride channel[J].Nature,1997,390(6658):417 -21.
  • 8Weylandt K H,Valverde M A,Nobles M,et al.Human ClC-3 is not the swelling-activated chloride channel involved in cell volume regulation[J].J Biol Chem,2001,276 (20):17461 -7.
  • 9Zhou J G,Ren J L,Qiun Q Y,et al.Regulation of intracellular Cl-concentration through volume-regulated ClC-3 chloride channels in Al0 vascular smooth muscle cells[J].J Boil Chem,2005,280(8):7301 -8.
  • 10Ryer V J,Sakakibara K,Wang C,et al.Protien kinase C delata indnces apoptosis of vascular smooth muscle cells through inducection of the tumor suppressor p53 by both p38-dependent and p38depenetment and p38-indepedentment mechanisms[J].J Biol Chem,2005,280(42):35310-7.

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同被引文献22

  • 1李景田,职君利,陈秀琴,刘微,崔宇,冯鉴强.Survivin在过氧化氢预处理诱导的适应性细胞保护中的作用[J].解剖学研究,2006,28(3):213-217. 被引量：2
  • 2李景田,唐小卿,陈秀琴,职君利,崔宇,刘微,冯鉴强.iNOS和COX-2在过氧化氢预处理诱导的适应性细胞保护中的作用[J].中国药理学通报,2007,23(2):219-223. 被引量：4
  • 3Wang C Y,Mayo M W,Korneluk RG,et al NF-κB antiapoptosis: induction of TRAF1 and TRAF2 and c-IAP1 and c-IAP2 to suppress caspase-8 activation [ J ]. Science, 1998 ;281 : 1680 - 3.
  • 4Tang X Q, Feng J Q, Chen J,et al. Protection of oxidative preconditioning against apoptosis induced by H2O2 in PC12 cells: Mechanism via MMP,ROS,and Bcl-2[J]. Brain Res ,2005 ;1057 :57 -64.
  • 5Wang C Y ,Mayo M W,Baldwin A S,et al. TNF- and cancer therapy-induced apoptosis : potentiation by inhibition of NF-κB [ J ]. Science, 1996,274:784 - 7.
  • 6Van Antwerp D J, Martin S J, Kafri T, et al. Suppression of TNFa- induced apoptosis by NF-κB [ J ]. Science, 1996,274:787 - 9.
  • 7Nakai M, Qin Z H, Chen J F, et al. Kainic acid-induced apoptosis in rat striatum is associated with nuclear factor-kappaB activation [ J ]. J Neurochem ,2000,74:647 - 58.
  • 8Zhu L, Fukuda S, Cordis G, et al. Anti-apoptotic protein survivin plays a significant role in tubular morphogenesis of human coronary arteriolar endothdial cells by hypoxic preconditioning [ J ]. FEBS Let ,2001 ;508:369 - 74.
  • 9Satoh T, Numakawa T, Abriru Y, et al. Production of reactive oxygen species and release of L-glutamate during superoxide anionmediated cell death of eerebellar ganule neurons[ J]. J Neurochem, 1998 ;70:316 - 24.
  • 10张海宁,丘钦英,关永源.ClC-3氯通道对Thapsigargin触发的Ca2＋运动的影响[J].中国药理学通报,2008,24(8):1057-1061. 被引量：2

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中国药理学通报
2007年 第9期

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