四种花色一串红RAPD分析

RAPD Analysis in the Four Kinds of Color-flowered Salvia splendens Ker-Gawl

以4种花色一串红为试材进行了RAPD研究,筛选出的RAPD最佳反应体系为:采用25μL的反应体系,其中含有2μL的10×buffer,0.45 mmol.L-1的dNTPs,2.0mmol.L-1的Mg2+,2U的TagDNA聚合酶,0.30μmol.L-1的引物,40 ng的模版。共筛选出12个特异引物,扩增出84条带,具有特异性的谱带为28条,占总带数的33%,在聚类图分析中,把4种花色一串红划分为两大类:首先是红色和红白双色聚为一类,然后和紫色聚在一起,白色单独为一类。

Four kinds of Color-flowered Salvia splendens Ker-Gawl were analyzed using RAPD. The results showed that the optimal RAPD reaction conditions were as follows： The optimal reaction mix- ture（25 μL total volume）contained 2.0 μL 10 ×buffer, 0.45 mmol· L^-1 dNTPs, 2.0 mmol· L^-1 Mg^2＋ , 2U Taq DNA polymerase, 0.30 μmol · L^-1 primer and 40 ng genomie DNA, 84 bands were amplified from 12 primers used, the differential bands had 28 bands, which account for 33 % of total bands. In cluster group analysis, the four kinds of color-flowered were divided into 2 styles： at the first, the red color and red-white color were grouped together, then they grouped with purple color into one cluster and the white color was single.