裂褶菌F17对偶氮染料刚果红的脱色降解及其产物分析

Decolorization and degradation of Congo Red by Schizophyllum sp.F17 and catabolite analysis

利用本实验室新构建的染料脱色降解体系,对裂褶菌F17(Schizophyllum sp.F17)脱色降解刚果红进行了研究,分析了该菌的主要降解酶,并对刚果红降解产物进行分离和鉴定.结果表明,裂褶菌F17在此体系中对刚果红表现出较高的脱色降解能力,菌球加入48h后脱色率达到91.5%;酶活检测表明,该菌主要产生锰过氧化物酶(MnP),并在脱色48h时,MnP酶活达到最大值96.1U·L-1.此外,对脱色96h和192h后的脱色液进行紫外-可见扫描,发现刚果红在可见光区495nm处的吸收峰已消失,并在紫外区出现多个吸收峰.通过高效液相色谱分离得到1种刚果红降解产物,用质谱和傅立叶红外光谱鉴定,发现该产物相对分子量为184.2,主要官能团为—C6H4—和芳基—NH2,结合刚果红结构和该产物的核磁共振波谱推断其为联苯胺;并且随着降解时间的延长,联苯胺逐渐被降解.

Congo Red（CR） was decolorized and degraded by the main enzymes secreted by Schizophyllum sp.F17 which were investigated in a novel system established by our laboratory.Catabolite analysis showed that CR was decolorized and degraded effectively by Schizophyllum sp.F17.The decolorization percentage of CR reached 91.5% 48 h after mycelial pellet addition and the maximum activity of MnP,the main degradation enzyme,reached 96.1 U·L^-1.Furthermore,from the UV-Vis spectrum of CR,the absorption peak at 495 nm vanished and new absorption peaks appeared in the ultraviolet region after 96 h and 192 h of decolorization.Benzidine is initially produced in the degradation process.It was isolated by HPLC and its identity was confirmed by FT-IR,mass spectrometry and NMR.The benzidine was itself degraded gradually over time.