硝基苯灌胃致小鼠蛋白质氧化损伤作用的研究

Study on protein oxidative damage in mice by intragastric administration of nitrobenzene

为了探讨硝基苯对小鼠多器官的蛋白质氧化损伤程度,采用不同剂量的硝基苯对小鼠进行灌胃染毒(每天1次,共30 d),用2,4二-硝基苯肼比色法测定小鼠组织蛋白质羰基含量,用以判断蛋白质的损伤程度.结果显示,各组织空白对照和油对照组间差异均不显著(p>0.05),随着染毒剂量的增加,肝脏、肾脏、脾脏和心脏的蛋白质羰基含量呈上升趋势,脑组织只在高剂量组表现明显升高趋势;低剂量组与油对照组相比,只有肝脏蛋白质羰基含量显著性升高(p<0.05);中、高剂量组与油对照组相比,各组织蛋白质羰基含量显著性升高(p<0.05).以上结果表明,低剂量硝基苯可以对细胞蛋白质产生氧化损伤作用,并且随着染毒剂量的增加,小鼠各组织蛋白质羰基含量呈上升趋势,蛋白质氧化损伤程度加重.

In order to explore the degree of protein oxidative damage in mice organs induced by nitrobenzene,different concentrations of nitrobenzene were administered by daily intragastric administration for 30 days.The protein carbonyl content was assayed with 2,4-dinitrophenylhydrazine to evaluate the degree of protein oxidative damage.No significant difference was observed between the control group and the solvent-only group（p〉0.05）.As the nitrobenzene concentration increased,the protein carbonyl content in liver,kidney,spleen and heart also increased,but in brain tissue,the increase was only observed in the high-dose group.In liver,the difference between the low-dose group and the control group was significant and the differences between middle or high dose groups and the solvent group were significant（p〈0.05）.In conclusion,low-doses of nitrobenzene caused oxidative damage to some mice tissues and damage to each tissue was greater at higher doses,but the sensitivity to nitrobenzene was different for each tissue.