骨髓干细胞和脂肪干细胞对纳米磁铁颗粒的摄取性能研究

Cellular Uptake of Magnetic Nanoparticles by Bone Marrow Stem Cells and Adipose Derived Stem Cells

目的研究两种纳米磁性氧化铁颗粒分别与骨髓干细胞(Bone marrow stem cells,BMSCs)和脂肪干细胞(Adipose derived stem cells,ADSCs)共培养时细胞对磁铁颗粒的摄取情况,以初步探讨其作为磁共振成像(MRI)对比剂去标记组织工程种子细胞而进行MRI示踪的可行性。方法制备粒径为6nm的Fe3O4磁性颗粒及经聚L-乳酸(PLLA)表面修饰的Fe3O4磁性颗粒(200nm),分别在细胞接种时及接种后24小时两种方式加入。使用电感耦合等离子体质谱(ICP-OES)检测细胞对铁颗粒的摄取量。结果细胞对铁颗粒的摄取量受纳米磁铁颗粒的种类,加入方式和细胞类型的影响。BMSCs和ADSCs对6nm粒径磁铁颗粒的摄取量显著高于对200nm铁颗粒的摄取量,而ADSCs摄取的量又高于BMSCs。结论6nm粒径的磁性氧化铁颗粒可以较好的被BMSCs和ADSCs吸收,具有良好的生物相容性,有望成为组织工程种子细胞的标记物来进行MRI示踪。

Objective This manuscript focused on the study of cellular uptake of magnetic nanoparticles （MNs） by bone marrow stem cells （BMSCs） and adipose derived stem cells （ADSCs） when the MNS were cultured together with those cells, respectively. The results obtained would then substantiate our hypothesis that MNs could be used as a contrast agent for seed cells of tissue engineering. Methods Fe3O4 magnetic nanoparticles of 6nm were prepared by synthesizing in high- temperature solution phase reaction. Inductively coupled plasma-mass spectroscopy （ICP-OES） was carried out to measure the cellular uptake of MNs. Results The cellular uptake of MNs was influenced by characteristics of MNs, addition style of MNs and cell types. Both BMSCs and ADSCs have the significantly higher intake amount of 6nm Fe than those of 200nm Fe particles, while the amount of Fe taken by ADSCs further exceeded those by BMSCs. Conclusion The willingness of BMSCs and ADSCs to uptake Fe3O4 of 6nm endowed such MNs with the potential application as a promising contrast agent for cells to undergo in vivo MRI assay.