摘要
对灯盏花素对大鼠急性脊髓损伤的干预和治疗作用进行了观察,以期为灯盏花素用于治疗脊髓损伤提供初步依据。取成年健康雄性SD大鼠52只,采用Allen重物打击法制作大鼠脊髓损伤模型,按随机分组的原则,分对照组和灯盏花素治疗组,治疗组按每天5mg·kg-1的总量分2次腹腔注射灯盏花素,对照组腹腔注射等量生理盐水。取损伤段脊髓组织,荧光免疫组化观察各组在不同时间点PAPP-1和Bcl-2表达变化,TUNEL法检测细胞凋亡并进行形态学观察。结果显示,在不同时间点,治疗组PAPP-1表达低于对照组,差异有显著性(P<0.05),Bcl-2表达量高于对照组,差异有显著性(P<0.05)。细胞凋亡数目TUNEL法治疗组低于对照组(P<0.05)。神经元及神经纤维变性、坏死轻于对照组。表明灯盏花素可抑制大鼠脊髓损伤后过氧化损伤和细胞凋亡,对继发性脊髓损伤起到保护作用。
To provide a preliminary basis for breviscapine to treat spinal cord injury (SCI) through observing it used to interfere in and treat SCI. 52 SD rats, ripe, healthy, male, were chosen to make models with SCI by Allen’s crush method, and then divided into two groups: breviscapine injection group (5mg·kg-1,twice, breviscapine supplied through intraperitoneal injection every day) and control group (same dose of normal saline, NS). Pieces of injuried myeloid tissue were taken, changes of the expression of PARP-1 and Bcl-2 in different times of two groups were observed through fluor and immunity method, apoptosis was detected by TUNEL and observed morphologically. Results in breviscapine group, the expression of PARP-1 was lower (P<0.05),while that of bcl-2 protein expression was higher than in control group(P<0.05). The number of apoptotic nerve cells was significantly less in breviscapine group than that in control group by TUNEL(P<0.05). The nerve cell and degeneration and necrosis of neurofibra were milder than in control group. It showed breviscapine could restain hyperoxidative damage and apoptosis after SCI in rats and protect neurons.
出处
《中医正骨》
2007年第9期1-3,共3页
The Journal of Traditional Chinese Orthopedics and Traumatology
