三七总皂甙对鼠脊髓损伤早期病理变化影响的观察

Histopathologic assessment of panax notoginseng saponins in rat spinal cord injury

Allen氏法250g·cm致伤60只Wistar大鼠T13～L1脊髓节段。伤后30min、2h、4h、第二及第三个24h分别腹腔注射三七总皂甙（PNS）100mg／kg及50mg／kg体重；另设二甲亚砜对照组及空白对照组。伤后30min、2h、6h、24h、1周及6周各取伤区脊髓组织送光、电镜检查。结果显示PNS治疗组脊髓灰质出血坏死较轻，周围白质大部存留，髓鞘残留，大量毛细血管和少突胶质细胞增生。提示PNS可减轻脊髓损伤后的灰质坏死，调节微循环和减轻继发性病理损害，为白质存活创造条件。

Experimental spinal cord injury(SCI) model was made by Allen's technique- Panax notoginseng saponins (PNS) was injected (50~ 100mg/kg ) intra peritoneally at 30min, 2h, 4h, the second and the third day after trauma. Pathological changes were compared with that of dimethyl sulfoxide in the control at 30min, 2h, 6h, 24h, 1 week and 6 weeks after impact by light and electron microscopy.The result revealed the less hemorrhage and necrosis of the grey matter. The surrounding white mater survived,capillaries and oligodendrocytes abundant proliferated. It showed PNS improved the spinal cord microcirculation,decreased the secondary pathological damage markedly and protected the white matter in early stage of SCI.