摘要
目的:研究淫羊藿甙对体外培养的大鼠成骨细胞功能的影响,探讨淫羊藿甙促进成骨的细胞及分子机制。方法:实验分组将不同浓度的淫羊藿甙加入到其中进行共培养,其对于成骨细胞增殖和分化的影响通过MTT比色实验、BGP含量的放免法检测以及用荧光二抗的免疫组化实验测定Ⅰ型胶原的表达来进行。结果:淫羊藿甙能促进成骨细胞Ⅰ型胶原的表达和BGP的合成。结论:淫羊藿甙具有刺激体外培养成骨细胞增殖、分化和矿化的功能。
Objective：To study the effects of Icariin injection on rat osteoblasts in vitro and to explore its possible cellular and molecular mechanisms in promoting the bone growth. Methods.. The rat osteoblasts cultured in vitro were stimulated with different concentration of Icariin according to the protocol. MTT, PNPP and ARS were used to observe the proliferation, activity of ALP and the number of mineral nodes of osteoblasts cultured in vitro. Results： It was found that Icariin can stimulate the proliferation, differentiation, maturation and mineralization of cultured osteoblasts in vitro. In proper concentration, Icariin could significantly promote the expression of mRNA, type Ⅰ collagen in rat osteoblasts in vitro. The expression levels of them increased with concentration of Lcariin. Conclusion： Lcariin had the effects on stimulating the proliferation, differentiation and maturation and mineralization of cultured osteoblasts.
出处
《中国中医骨伤科杂志》
CAS
2007年第9期36-38,共3页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
