抗凋亡基因GW112在胃癌组织中的表达及其临床意义

Expression of Antiapoptotic-related gene GW112 in Gastric Cancer Tissue and Its Clinical Significance

目的:观察抗凋亡基因GW112(Olfactomedin 4)在胃癌组织的表达水平及临床意义。方法:采用SYBR Green I实时定量RT-PCR检测25例胃癌组织及胃癌外周正常胃组织中GW112的表达,并分析其表达情况与临床病理资料的相关性。结果:GW112基因在胃癌组织的表达水平(M=0.12)显著高于正常胃组织(M=0.02,P<0.05),在有淋巴结转移的胃癌组织中的表达(M=0.23)显著高于无淋巴结转移的胃癌组织(M=0.04,P<0.05),在肿瘤TNM分期Ⅲ/Ⅳ期的胃癌组织(M=0.23)的表达显著高于Ⅰ/Ⅱ期(M=0.04,P<0.05);在年龄高(>60岁)患者的胃癌组织中的表达(M=0.25)高于年龄低(≤60岁)的患者(M=0.06,P<0.05)。在性别和分化程度上差异没有统计学意义(P>0.05)。结论:GW112基因在胃癌组织中高表达,与胃癌的侵袭和转移相关,可作为胃癌侵袭、转移的标志物。

Objective：To investigate the expression levels of antiapoptotic-related gene GW112 in gastric cancer and control tissues and its clinical significance. Methods： GW112 expression levels were detected by SYBR Green Ⅰ quantitative real-time PCR in cancer and normal tissues surrounding the cancer in 25 patients. Analysis was performed to study the correlation between the expression of GW112 and clinicopathological parameters. Results： Expression levels of GW112 were significantly higher in gastric cancer tissues than in normal tissues （ M =0.12 vs M =0.02, P 〈 0.05）. The expressions of GW112 in the lymph node invasive group were significantly higher than that of non- lymph node invasive group（ M = 0.23 vs M = 0.04 ,P 〈 0.05） . Overexpression of GW112 was also found more frequently in stage m/iv cancer group（ M = 0, 23 ）than in stage I/II group （M = 0.04） . In addition, the expressions of GW112 mRNA in gastric cancer tissues from elder patients （ 〉 60 year） were increased significantly compared with that in younger patients （≤〈60 year） （M = 0.25 vs M = 0.06）. GW112 expression was not correlated with the sex and histological grades of cancer （ P 〉 0.05 ）. Conclusion： GW112 was highly expressed in gastric cancer tissues. The expression levels were positively correlated with the degree of malignancy, suggesting its use as a biomarker for invasiveness and metastasis of grastric cancer.