核酸酶BN基因的克隆及序列分析

Cloning and Sequencing of the Ribonuclease (Barnase) Gene

从解淀粉芽抱杆菌（Bacillusamyloliquefaciens）中提取染色体DNA经过PCR扩增得到Barnase（核酸酶BN）基因，然后克隆到质粒PGEM-7Zf(+)的Smal位点上并进行序列分析。结果表明．核酸酶BN基因的核着酸序列与已报道的序列相比具有99．7％的同源性，长度为336bp，根据核苷酸序列推断的氨基酸序列则完全一致。该工作为以后利用核酸酶的特异表达来获得雄性不育植物打下了基础。

Chromosomal DNA was isolated from the Bacillus amyloliquefaciens, and Barnase gene was amplified by PCR, then cloned and sequenced at the SmaI site of plasmid PGEM- 7zf(+ ). The result found that the nucleotide sequence with 336 bp in length had a homology of 99 - 7 % as com-pared with the reported sequence. Amino acid sequence was produced according to the nucleotide sequence. Our study makes a future contribution to producing male sterile transgenic plants by using Barnas gene.