淡水养殖鱼类主要细菌性传染病快速诊断的研究

Study on the Rapid Diagnosis to the Main Bacterial Disease in the Cultured Freshwater Fishes Ⅲ Direct Fluorescent Antibody Technique for Detection of Six Strainspathofenic Bacterial of Fishes

用６种淡水鱼常见致病菌制备了荧光抗体诊断液，经交叉染色及对照菌染色，表明具有较高的特异性，可直接用病鱼的内脏涂片对致病菌做出鉴定，具有直观、快速的特点，适用于鱼类细菌病的快速诊断及流行病学调查。

A bacterial diagnosis solution of staphylococcus protein A(SPA) was prepared by us ing a SPA international standard bacterial strainCowan I labelled with super antisera to 16 strains of the motil eaeromonas species in freshwater fishes,and a SPA Co agglutination test(SPA CoA)was established for rapid detection of the main bacterialdiseases in freshwater fishes.The results of detection could bereported tithin 3 min by SPA CoA agter sample was cultivated on agarplates for 16～18 h.The sensitivity of this test was 25 ～ 50 timeshigher than that of slide agglutination,and the specitivity of the tests how no cross reaction and repeat well.The presented test was proved to besimple,specific,sensitive and timesaving,and to be used i thediagnosis and epidemiologic survey of bacterial diseases in freshwaterfishes.The sensitivity test to the 11 sorts of medicine showed anevidence for precaution and therapy.