小肠部分切除大鼠术后一氧化氮代谢途径干预对葡萄糖代谢的影响

Influence of nitric oxide metabolism approach on glucose metabolism in rats after small intestine resection

目的:观察小肠部分切除大鼠术后一氧化氮代谢途径干预对大鼠术后葡萄糖代谢的影响,寻找解决术后胰岛素抵抗的方法。方法:实验于2005-01/2006-08在解放军第四军医大学第二附属医院中心实验室完成。实验分组:96只大鼠随机数字表法分为4组,正常对照组,L-硝基-精氨酸甲酯处理组,L-精氨酸处理组,L-精氨酸+L-硝基-精氨酸甲酯处理组,每组24只。实验干预:建立大鼠小肠部分切除模型,各干预组分别尾静脉注射1mg/kgL-硝基-精氨酸甲酯、100mg/kgL-精氨酸处理、0.5mg/kgL-硝基-精氨酸甲酯+50mg/kgL-精氨酸处理进行一氧化氮代谢途径干预。正常对照组只注射1mL生理盐水。实验评估:①给药后2,8,12,24,48h取血,测定血清中一氧化氮含量及血糖检测。②给药后24h,48h,1周后采用胰岛素敏感性实验计算每公斤代谢体质量每分钟代谢葡萄糖的量。结果:96只大鼠均进入结果分析。①血清一氧化氮含量:L-硝基-精氨酸甲酯处理组各时间点血清一氧化氮含量低于正常对照组(P<0.05),而L-精氨酸处理组各时间点血清一氧化氮含量高于正常对照组(P<0.05);L-精氨酸+L-硝基-精氨酸甲酯处理组与正常对照组比较差异无显著性(P>0.1)。②空腹血糖:给药后8,12,24,48hL-硝基-精氨酸甲酯处理组高于正常对照组(P<0.05)。给药后8,12,24,48hL-精氨酸处理组低于正常对照组(P<0.05);L-精氨酸+L-硝基-精氨酸甲酯处理组与正常对照组比较差异无显著性(P>0.1)。③稳态情况下每千克代谢体质量每分钟代谢葡萄糖的量:L-硝基-精氨酸甲酯处理组显著低于正常对照组(P<0.05),而L-精氨酸处理组则显著高于正常对照组(P<0.05);L-精氨酸+L-硝基-精氨酸甲酯处理组与正常对照组比较差异无显著性(P>0.1)。结论:小肠部分切除大鼠术后一氧化氮代谢途径在葡萄糖转运和胰岛素活性方面可能起着重要作用,提示其可能是解决术后胰岛素抵抗的一种方法。

AIM： To observe the influence of nitric oxide metabolism on glucose metabolism in rats after small intestine resection and search for methods to solve postoperative insulin resistance. METHODS： The experiment was performed at the Central Laboratory of Second Affiliated Hospital of Fourth Military Medical University of Chinese PLA from January 2005 to August 2006. Experimental groupings： Totally 96 rats were divided into 4 groups by random digits table, namely normal control group, NG-nitro-L-arginine methyl ester （L-NAME） group, L-arginine （L-Arg） group and L-Arg plus L-NAME group with 24 in each group. Experimental intervention： Small intestine resection rat models were established. Rats in each group received nitnc oxide metabolism intervention by injecting 1 mg/kg L-NAME, 100 mg/kg L-Arg, 0.5 mg/kg L-NAME plus 50 mg/kg L-Arg in caudal vein. Rats in the normal control group only received 1 mL saline. Experimental evaluation： （1）Bloed was collected at hours 2, 8, 12, 24 and 48 after administration, and then content of nitric oxide and glucose in serum were determined. （2）insulin sensitivity test was performed 24 hours, 48 hours and 1 week after administration. RESULTS： A total of 96 rats were involved in the result analysis. （1）Content of nitnc oxide in serum： The content in L-NAME group was lower than that in the normal control group （P 〈 0.05）. The content in L-Arg group was higher than that in the normal control group （P 〈 0.05）. There was no significant difference between the L-Arg plus L-NAME group and normal control group （P 〉 0.1）. （2）Fasting glucose： The level was higher in the L-NAME group than the normal control group （P 〈 0.05）, but the level was lower in the L-Arg group than the normal control group（P 〈 0.05）8, 12, 24 and 48 hours after administration. There was no significant difference between the L-Arg plus L-NAME group and the normal control group （P 〉 0.1）. （3）Content of glucose metabolism in a minute of a kilogram body mass： The content was obviously lower in the L-NAME group than the normal control group （P 〈 0.05）, but the content was markedly higher in the L-Arg group than the normal control group （P 〈 0.05）. There was no significant difference between the L-Arg plus L-NAME group and the normal control group （P 〉 0.1）. CONCLUSION： Nitnc oxide metabolism pathway plays important roles in glucose transport and insulin activity in rats after small intestine resection, it indicates that nitric oxide metabolism may be a method of solving postoperative insulin resistance.