自然孵化与换壳培养鸭胚胎碱性磷酸酶活性的研究

Study on the Activity of Alkaline Phosphatase in Duck Embryos with Normal Incubation and Culture in Surrogate Eggshells

为了探讨碱性磷酸酶活性(ALP)对鸭胚胎钙代谢的影响,分别测定了自然孵化(对照组)和换壳培养(试验组)至14d、16d、18d、20d、22d、24d、26d、28d鸭胚胎腿骨和血浆中的ALP活性和腿骨中ALP比活性。鸭胚胎腿骨和血浆中ALP活性随着胚胎日龄的增加迅速上升(P<0.01),同一日龄的ALP活性实验组均显著高于对照组(P<0.01)。腿骨中ALP比活性的变化与ALP活性相似,第22d以后实验组显著高于对照组(P<0.01)。结果表明,骨组织和血浆中ALP活性以及腿骨组织中ALP比活性可以在一定程度上反映鸭胚胎发育过程中钙的代谢情况;鸭胚胎体外培养缺钙现象主要发生在胚胎培养的后期。

The effect of alkaline phosphatase （ALP） on calcium metabolism of duck embryo was studied in present experiment The activity of ALP in the bone and plasma and the specific activity in the bone of em- bryos was tested at the incubated day of 14th, 16th, 18th, 20th, 22nd, 24th, 26th, 28th in the surrogate eggshell cultured group （treatment） and normal one （control）. The result showed that the activity of ALP increased significantly from the day of 14th to the day of 28th （P〈0.01） both in treatment and in control, but the activity was significantly higher in the former than the latter with the same cultured time （P〈0.01）. The specific activity in the bone of embryos was higher in treatment than in control after 22nd, like the variation of ALP activity. The result indicated that activity of ALP in the bone and plasma could elucidate the calcium metabolism of the embryos and showed that calcium deficiency was appeared in the later development period of embryo in the surrogate eggshell group.