一种快速低廉的PCR探针标记方法

A Rapid and Twopenny PCR Labeling Method

【研究目的】探针标记技术是分子生物学和基因工程研究中常用实验技术,需要发展高效、快速、低成本、安全、简捷的DNA探针标记方法;【方法】利用普通的Taq酶进行PCR探针标记,电泳和Southern杂交检测探针标记结果;【结果】电泳检测发现标记产物在电泳时相对非标记的对照表现明显滞后,表明探针标记成功;电泳条带清晰明亮,表明探针标记效率高;Southern杂交条带清晰,说明该方法标记的探针可用于Southern等分子杂交实验;【结论】利用普通的Taq酶进行PCR探针标记,是一种低成本、高效、快速的DNA探针标记方法。

[OBJECTIVE] DNA labeling technology is important for research of molecular and genetic engineering, So it is important to develop a high-efficiency, rapid, lowcost, safe, simple and convenient method for labeling DNA; [METHOD] Based on PCR principle, DNA was labeled using Taq DNA Polymerase and the result was detected by electrophoresis and southern-blot.[RESULTS]The electrophoresis results indicated the product labeled was lag than control DNA, which improved that the label succeed; The bright and clear strip indicated the labeling efficiency was high; Cross strip in southern-blot was clear, which proved the product labeled by the method can be used in molecular hybridization. [CONCLUSIONIPCR labeling using Taq DNA polymerase is a low-cost, high-efficlency and rapid DNA labeling method.