摘要
地黄花叶病毒(Rehmannia mosaic virus,ReMV)是地黄上发生的一种烟草花叶病毒属(Tobamovirus)可能的新病毒。笔者对ReMV的生物学特性进行了初步研究。结果表明,ReMV粒子形态为杆状,可以侵染茄科、番杏科、十字花科和藜科等4科10种植物,其中在茄科的心叶烟、黄花烟、蔓陀罗;番杏科的番杏;藜科的苋色藜和昆诺藜上表现为局部枯斑症状。在红花烟、普通烟、番茄和白菜等植物上表现为系统花叶症状。ReMV的钝化温度为90-95℃,稀释限点为10-5-10-6,20-22℃条件下的体外存活期在60d以上。
Rehmannia mosaic virus (ReMV) is probably a new species of Tobamovirus. The biological char- acteristics of ReMV were detected. The results revealed that the ReMV particle is rod-shaped and it could infect 10 plant species in 4 families. ReMV causes necrotic lesions on hosts such as Nicotiana glutinosa, Nicotiana rustica, Datura stramonium, Chenopodium quinoa, Chenopodium amaranticolor and Tetragonia expansa and causes systemic mosaic on hosts such as Nicotiana tabacum L, Nicotiana tobacum CV. K326, Lycoporsicon .esculentum and Brassica pekinensis. Its thermal inactivation point (TIP) ranges from 90℃ to 95℃, the dilution end point (DEP) is 10-5-10-6 and the longevity in vitro (LIV) was more than 60 days in 20-22℃.
出处
《中国农学通报》
CSCD
2007年第9期406-408,共3页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金项目"地黄花叶病毒(ReMV)基因组结构
功能与进化关系研究"(30571217)和"地黄花叶病毒(ReMV)致病性相关基因研究"(30671356)资助
关键词
地黄花叶病毒
提纯
电镜观察
生物学特性
Rehmannia mosaic virus, Purification, Electron microscopy observation, Biological characteris-tics