中药复方肺瘤平膏对树突状细胞功能影响的拆方研究

Influence of Feiliuping Extractum and its modified formulas on function of dendritic cells

目的观察肺瘤平膏及其拆方干预体外培养体系中树突状细胞(DC)抗原递呈功能的作用并探讨其分子机制。方法建立体外人外周血单个核细胞(PBMC)诱导分化成熟DC的诱导培养体系,从健康人外周血中分离获得PBMC,采用多种细胞因子(TNFα、IL-4、GM-CSF)联合诱导,获得了分化与功能相对成熟的DC,用流式细胞仪检测肺瘤平膏及其拆方含药血清干预DC与抗原递呈功能相关表面分子表达,及其对DC分泌IL-12含量的影响,进行初步研究。结果肺瘤平膏可上调DC与抗原递呈功能相关膜分子MHC-Ⅱ、CD80、CD83、CD86及CD40的表达,并促进DC分泌IL-12含量。结论肺瘤平膏能够调节DC抗原递呈功能,提高机体的抗肿瘤免疫监视功能。

Objective To observe the interference of Feiliuping Extractum and its modified formulas on the antigen presentation of dendritic cells in cultural system in vitro and study the molecular mechanism. Methods The inducing cultural system was established to induce and differentiate mature dendritic cells in human peripheral blood mononuclear cells （PBMC） , ie. PBMC were separated from peripheral blood of healthy people. The cytokines （ TNFα, IL-4 and GM-CSF） were used jointly to induce and obtain the mature dendritic cells in differentiation and function. The interference of Feiliuping Extractum and its modified formulas to dendritic cells and the expressions of related surface molecules to antigen presentation were detected by using flow cytometry, and their influence on the content of IL-12 secreted by dendritic cells were studied. Results Feiliuping Extractum up-regulated the expressions of dendritic cells and related cytomembrane molecules to antigen presentation, such as MHC-Ⅱ, CD80, CD83, CD86 and CD40 and promoted the IL-12 content secreted by dendritic cells. Conclusion Feiliuping Extractum can regulate antigen presentation of dendritic cells and improve the body function of anti-tumor immunological surveillance.