二精丸总多糖抗长期应激大鼠学习记忆障碍的作用机制

Mechanism of Total Polysaccharides in Erjing Pill on Long-term Stress-induced Cognitive Deficits

目的:探讨二精丸总多糖抗长期应激大鼠学习记忆障碍的作用机制。方法:采用长期激怒应激法造模,二精丸总多糖灌胃,给药结束后用Morris水迷宫法测定各组实验大鼠的学习记忆能力;采用酶联免疫法测定大鼠血清雌二醇(E2)、睾酮(T)、皮质酮(CORT)、E2/T值以及海马部位乙酰胆碱酯酶(AchE)含量。结果:二精丸总多糖组与模型组相比,平均潜伏期明显缩短(P<0.05),搜索方式明显改变,游泳路径显示在中环区和中心区百分比增加(P<0.05),平台区穿越次数增多(P<0.05);E2和E2/T值明显降低(P<0.05),T和CORT明显升高(P<0.05);海马部位AchE浓度明显减少(P<0.05)。结论:二精丸总多糖能提高长期激怒应激法所致肾阴虚模型大鼠学习记忆能力,同时降低E2和E2/T浓度;升高T和CORT浓度;抑制AchE活性。

Objective： To study the mechanism of total polysaccharides in Erjing Pill on long-term stress-induced cognitive deficits. Methods： Model rats of kidney-YIN deficiency were made by using the method of long-term stres.s, Morris water maze was used to determine the cognition ability of different rats, then we used euzymelinked imrnunosorbent assay to determine the content of E2, T, CORT, E2/T in serum and AchE in hippocampus（HIP）. Results：Compared to model group, total polysaccharides in Erjing Pill can shortened obviously mean incubation period（ P〈 0.05）and changed obviously the way of searching, it increased obviously the sector percentage distribution of swimming pathway disclosed in middle ring zone and center zone（P 〈 0.05）and the frequency of passing through plateau region（P〈 0.05） ; it Lowered obviously the content of E2 and Ee/T（P〈0.05）,it heightened obviously the content of T and CORT（ P 〈 0.05 ）, meanwhile it also shortened the activity of AchE, in HIP（ P 〈 0.05 ）. Conclusion： Total polysaccharides in Erjing Pill could elevate the cognition ability of model rats, lowering the content of E2 and E2/T, heightening the content of T and CORT, restraining the activity of Ache in HIP.